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Cellular internaliz...
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Kilk, KalleStockholms universitet,Institutionen för neurokemi och neurotoxikologi,University of Tartu, Estonia
(author)
Cellular internalization of a cargo complex with a novel peptide derived from the third helix of the islet-1 homeodomain : Comparison with the penetratin peptide
- Article/chapterEnglish2001
Publisher, publication year, extent ...
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2001-11-02
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American Chemical Society (ACS),2001
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:su-23348
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-23348URI
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https://doi.org/10.1021/bc0100298DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Cellular translocation into a human Bowes melanoma cell line was investigated and compared for penetratin and pIsl, two peptides that correspond to the third helices of the related homeodomains, from the Antennapedia transcription factor of Drosophila and the rat insulin-1 gene enhancer protein, respectively. Both biotinylated peptides internalized into the cells with similar efficacy, yielding an analogous intracellular distribution. When a large cargo protein, 63 kDa avidin, was coupled to either peptide, efficient cellular uptake for both the peptide−protein complexes was observed. The interactions between each peptide and SDS micelles were studied by fluorescence spectroscopy and acrylamide quenching of the intrinsic tryptophan (Trp) fluorescence. Both peptides interacted strongly and almost identically with the membrane mimicking environment. Compared to penetratin, the new transport peptide pIsl has only one Trp residue, which simplifies the interpretation of the fluorescence spectra and in addition has a native Cys residue, which may be used for alternative coupling reactions of cargoes of different character.
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Magzoub, MazinStockholms universitet,Institutionen för biokemi och biofysik
(author)
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Pooga, MargusStockholms universitet,Institutionen för neurokemi och neurotoxikologi,Estonian Biocenter, Estonia
(author)
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Eriksson, L. E. GöranStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)goran
(author)
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Langel, ÜloStockholms universitet,Institutionen för neurokemi och neurotoxikologi(Swepub:su)ulo
(author)
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Gräslund, AstridStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)astrid
(author)
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Stockholms universitetInstitutionen för neurokemi och neurotoxikologi
(creator_code:org_t)
Related titles
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In:Bioconjugate chemistry: American Chemical Society (ACS)12:6, s. 911-9161043-18021520-4812
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