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A novel cell-penetr...
A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids
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- EL Andaloussi, Samir (author)
- Stockholms universitet,Institutionen för neurokemi
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- Johansson, Henrik (author)
- Stockholms universitet,Institutionen för neurokemi
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- Holm, Tina (author)
- Stockholms universitet,Institutionen för neurokemi
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- Langel, Ülo (author)
- Stockholms universitet,Institutionen för neurokemi
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- English.
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In: Molecular Therapy. - : Elsevier BV. - 1525-0016 .- 1525-0024. ; 15:10, s. 1820-1826
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Cell-penetrating peptides (CPPs) have attracted increasing attention in the past decade as a result of their high potential to convey various, otherwise impermeable, bioactive agents across cellular plasma membranes. Albeit different CPPs have proven potent in delivery of different cargoes, there is generally a correlation between high efficacy and cytotoxicity for these peptides. Hence, it is of great importance to find new, non-toxic CPPs with more widespread delivery properties. We present a novel CPP, M918, that efficiently translocates various cells in a non-toxic fashion. In line with most other CPPs, the peptide is internalized mainly via endocytosis, and in particular macropinocytosis, but independent of glycosaminoglycans on the cell surface. In addition, in a splice correction assay using antisense peptide nucleic acid (PNA) conjugated via a disulphide bridge to M918 (M918-PNA), we observed a dose-dependent increase in correct splicing, exceeding the effect of other CPPs. Our data demonstrate that M918 is a novel CPP that can be used to translocate different cargoes inside various cells efficiently.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Pep tides
- Nucleic acids
- Proteins
- Cell membranes
- Endocytosis
- Chemistry
- Kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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