Structural determination of the O-antigenic polysaccharide from Escherichia coli O74

Jonsson, K. Hanna M.Stockholms universitet,Institutionen för organisk kemi (författare)

The structure of the O-antigen polysaccharide (PS) from Escherichia coli O74 has been determined. Component analysis, together with 1H and 13C NMR spectroscopy as well as 1H,15N-HSQC experiments were employed to elucidate the structure. Inter-residue correlations were determined by 1H,1H-NOESY and 1H,13C-heteronuclear multiple-bond correlation experiments. The PS is composed of tetrasaccharide repeating units with the following structure: Full-size image (5K) Cross-peaks of low intensity from an α-linked N-acetylglucosamine residue were present in the NMR spectra, and spectral analysis indicates that they originate from the penultimate residue in the polysaccharide. Consequently, the biological repeating unit has a 3-substituted N-acetyl-d-glucosamine residue at its reducing end. The 1H, 13C and 15N NMR chemical shifts of the α- and β-anomeric forms of d-Fucp3NAc are also reported. The repeating unit of the E. coli O74 O-antigen is identical to that of the capsular polysaccharide from E. coli K45.

Weintraub, AndrejKarolinska Institutet (författare)
Widmalm, GöranStockholms universitet,Institutionen för organisk kemi(Swepub:su)gw (författare)
Stockholms universitetInstitutionen för organisk kemi (creator_code:org_t)

Ingår i:Carbohydrate Research: Elsevier Ltd.344:12, s. 1592-15950008-62151873-426X

Av författaren/redakt...: Jonsson, K. Hann ...; Weintraub, Andre ...; Widmalm, Göran

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