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Platelet PAR1 recep...
Platelet PAR1 receptor density-Correlation to platelet activation response and changes in exposure after platelet activation
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- Ramström, Sofia, 1973- (author)
- Linköpings universitet,Klinisk kemi,Hälsouniversitetet
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- Vretenbrant-Öberg, Karin, 1979- (author)
- Linköpings universitet,Klinisk kemi,Hälsouniversitetet
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- Åkerström, Finn (author)
- Leicester Medical School University of Leicester, UK
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- Enström, Camilla, 1978- (author)
- Linköpings universitet,Klinisk kemi,Hälsouniversitetet
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- Lindahl, Tomas, 1954- (author)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Klinisk kemi
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(creator_code:org_t)
- Elsevier BV, 2008
- 2008
- English.
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In: Thrombosis Research. - : Elsevier BV. - 0049-3848 .- 1879-2472. ; 121:5, s. 681-688
- Related links:
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http://urn.kb.se/res...
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https://liu.diva-por... (primary) (Raw object)
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http://liu.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Introduction: A polymorphism (-14 A/T) affecting PAR1 expression on the platelet surface has recently been identified. A two-fold variation in receptor density, which correlated with the platelet response to PAR1-activating peptide (PAR1-AP), has been reported. Materials and methods: We used flow cytometry to measure the correlation between the number of PAR1 receptors and platelet activation. We also measured the changes in receptor exposure after platelet activation with PAR1-AP, ADP, PAR4-AP or a collagen-related peptide (CRP). Results: In our study, the PAR1 receptor number varied almost four-fold, from 547 to 2063 copies/platelet (mean ± S.D. 1276 ± 320, n = 70). The number of PAR1 receptors on resting platelets correlated to platelet fibrinogen binding and P-selectin expression following platelet activation with PAR1-AP (r2 = 0.30, p < 0.01 and r2 = 0.15, p < 0.05, respectively, n = 36). The correlation was not improved by exclusion of the ADP-component from the PAR1-AP-induced response. We found a trend, but no statistically significant differences in PAR1 receptor number and platelet reactivity between A/A individuals and T/A or T/T individuals. Ex vivo activation with PAR1-AP decreased PAR1 surface exposure to 71 ± 19% of the exposure on resting platelets (mean ± S.D., p < 0.01, n = 19), while activation by ADP, PAR4-AP or CRP significantly increased the exposure, to 151 ± 27%, 120 ± 21% and 138 ± 25%, respectively (n = 11, 11 and 10). Conclusions: This study shows a large variation in PAR1 receptor number in healthy individuals, a variation correlated to the platelet activation response. We found a significant reduction in PAR1 surface exposure after adding PAR1-AP, while activation with ADP, PAR4-AP or CRP increased the exposure.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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