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Crystal structure o...
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Massad, TariqStockholms universitet,Institutionen för biokemi och biofysik
(author)
Crystal structure of the P2 C-repressor : a binder of nonpalindromic direct DNA repeats
- Article/chapterEnglish2010
Publisher, publication year, extent ...
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2010-07-17
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Oxford University Press (OUP),2010
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:su-42003
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-42003URI
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https://doi.org/10.1093/nar/gkq626DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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As opposed to the vast majority of prokaryoticrepressors, the immunity repressor of temperateEscherichia coli phage P2 (C) recognizes nonpalindromicdirect repeats of DNA rather thaninverted repeats. We have determined the crystalstructure of P2 C at 1.8A ° . This constitutes the firststructure solved from the family of C proteins fromP2-like bacteriophages. The structure reveals thatthe P2 C protein forms a symmetric dimer orientedto bind the major groove of two consecutive turns ofthe DNA. Surprisingly, P2 C has great similarities tobinders of palindromic sequences. Nevertheless, thetwo identical DNA-binding helixes of the symmetricP2 C dimer have to bind different DNA sequences.Helix 3 is identified as the DNA-recognition motif inP2 C by alanine scanning and the importance for theindividual residues in DNA recognition is defined.A truncation mutant shows that the disorderedC-terminus is dispensable for repressor function.The short distance between the DNA-bindinghelices together with a possible interaction betweentwo P2 C dimers are proposed to be responsible forextensive bending of the DNA. The structure providesinsight into the mechanisms behind the mutants ofP2 C causing dimer disruption, temperature sensitivityand insensitivity to the P4 antirepressor.
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Skaar, KarinStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)kasa0238
(author)
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Nilsson, HannaStockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi(Swepub:su)hani0104
(author)
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Damberg, PeterStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)peterd
(author)
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Henriksson-Peltola, PetriStockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
(author)
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Haggård-Ljungquist, ElisabethStockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi(Swepub:su)hagga
(author)
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Högbom, MartinStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)hogbom
(author)
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Stenmark, PålStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)stenm
(author)
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Stockholms universitetInstitutionen för biokemi och biofysik
(creator_code:org_t)
Related titles
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In:Nucleic Acids Research: Oxford University Press (OUP)38:21, s. 7778-77900305-10481362-4962
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