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Increased fatigue r...
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Bruton, Joseph D.Karolinska Institutet
(författare)
Increased fatigue resistance linked to Ca(2+)-stimulated mitochondrial biogenesis in muscle fibres of cold-acclimated mice
- Artikel/kapitelEngelska2010
Förlag, utgivningsår, omfång ...
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2010-10-29
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Wiley,2010
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:su-45468
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-45468URI
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https://doi.org/10.1113/jphysiol.2010.198598DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:121619100URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Mammals exposed to a cold environment initially generate heat by repetitive muscle activity (shivering). Shivering is successively replaced by the recruitment of uncoupling protein-1 (UCP1)-dependent heat production in brown adipose tissue. Interestingly, adaptations observed in skeletal muscles of cold-exposed animals are similar to those observed with endurance training. We hypothesized that increased myoplasmic free [Ca2+] ([Ca2+]i) is important for these adaptations. To test this hypothesis, experiments were performed on flexor digitorum brevis (FDB) muscles, which do not participate in the shivering response, of adult wild-type (WT) and UCP1-ablated (UCP1-KO) mice kept either at room temperature (24 ºC) or cold-acclimated (4 ºC) for 4-5 weeks. [Ca2+]i (measured with indo-1) and force were measured under control conditions and during fatigue induced by repeated tetanic stimulation in intact single fibres. The results show no differences between fibres from WT and UCP1-KO mice. However, muscle fibres from cold-acclimated mice showed significant increases in basal [Ca2+]i (~50%), tetanic [Ca2+]i (~40%), and sarcoplasmic reticulum (SR) Ca2+ leak (~four-fold) as compared to fibres from room-temperature mice. Muscles of cold-acclimated mice showed increased expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) and increased citrate synthase activity (reflecting increased mitochondrial content). Fibres of cold-acclimated mice were more fatigue resistant with higher tetanic [Ca2+]i and less force loss during fatiguing stimulation. In conclusion, cold exposure induces changes in FDB muscles similar to those observed with endurance training and we propose that increased [Ca2+]i is a key factor underlying these adaptations.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Aydin, JanKarolinska Institutet
(författare)
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Yamada, Takashi
(författare)
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Shabalina, Irina G.Stockholms universitet,Avdelningen för fysiologi(Swepub:su)chaba
(författare)
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Ivarsson, NiklasKarolinska Institutet
(författare)
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Zhang, Shi-JinKarolinska Institutet
(författare)
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Wada, Masanobu
(författare)
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Tavi, Pasi
(författare)
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Nedergaard, JanStockholms universitet,Avdelningen för fysiologi(Swepub:su)neder
(författare)
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Katz, AbramKarolinska Institutet
(författare)
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Westerblad, HåkanKarolinska Institutet
(författare)
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Karolinska InstitutetAvdelningen för fysiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Physiology: Wiley588:21, s. 4275-42880022-37511469-7793
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Bruton, Joseph D ...
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Aydin, Jan
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Yamada, Takashi
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Shabalina, Irina ...
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Ivarsson, Niklas
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Zhang, Shi-Jin
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Wada, Masanobu
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Tavi, Pasi
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Nedergaard, Jan
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Katz, Abram
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Westerblad, Håka ...
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Journal of Physi ...
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Stockholms universitet
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Karolinska Institutet