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Assembly of a fragm...
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Crona, Mikael,1981-Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik,Britt-Marie Sjöberg
(författare)
Assembly of a fragmented ribonucleotide reductase by protein interaction domains derived from a mobile genetic element
- Artikel/kapitelEngelska2011
Förlag, utgivningsår, omfång ...
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2010-10-22
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Oxford University Press,2011
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:su-45737
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-45737URI
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https://doi.org/10.1093/nar/gkq924DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-41107URI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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authorCount :6
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Ribonucleotide reductase (RNR) is a critical enzyme of nucleotide metabolism, synthesizing precursors for DNA replication and repair. In prokaryotic genomes, RNR genes are commonly targeted by mobile genetic elements, including free standing and intron-encoded homing endonucleases and inteins. Here, we describe a unique molecular solution to assemble a functional product from the RNR large subunit gene, nrdA that has been fragmented into two smaller genes by the insertion of mobE, a mobile endonuclease. We show that unique sequences that originated during the mobE insertion and that are present as C- and N-terminal tails on the split NrdA-a and NrdA-b polypeptides, are absolutely essential for enzymatic activity. Our data are consistent with the tails functioning as protein interaction domains to assemble the tetrameric (NrdA-a/NrdA-b)2 large subunit necessary for a functional RNR holoenzyme. The tails represent a solution distinct from RNA and protein splicing or programmed DNA rearrangements to restore function from a fragmented coding region and may represent a general mechanism to neutralize fragmentation of essential genes by mobile genetic elements.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Sjöberg, Britt-MarieStockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik(Swepub:su)bsjob
(författare)
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Moffatt, ConnorDepartment of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario
(författare)
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Edgell, David R.Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario
(författare)
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Friedrich, Nancy C.Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario
(författare)
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Hofer, AndersUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)anho0002
(författare)
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Stockholms universitetInstitutionen för molekylärbiologi och funktionsgenomik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Nucleic Acids Research: Oxford University Press39:4, s. 1381-13890305-10481362-4962
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