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Development of a hi...
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Grey, CarlLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
(författare)
Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
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Elsevier BV,2009
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:su-59966
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-59966URI
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https://doi.org/10.1016/j.jchromb.2009.05.003DOI
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https://lup.lub.lu.se/record/1463105URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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authorCount :4
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In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Edebrink, Per
(författare)
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Krook, Maria
(författare)
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Jacobsson, Sven P.Stockholms universitet,Institutionen för analytisk kemi(Swepub:su)sjaco
(författare)
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BioteknikCentrum för tillämpade biovetenskaper
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of chromatography. B: Elsevier BV877:20-21, s. 1827-18321570-02321873-376X
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