Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides

• In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method. 

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology (hsv//eng)

Nyckelord

HPAEC-PAD

MALDI-TOF MS

Oligosaccharides

N-glycosylation

Glycoprotein

Monoclonal antibodies

Analytical chemistry

Analytisk kemi

Monoclonal antibodies

Glycoprotein

N-glycosylation

Oligosaccharides

HPAEC-PAD

MALDI-TOF MS

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