SwePub
Sök i LIBRIS databas

  Extended search

WFRF:(Gudise Santhosh)
 

Search: WFRF:(Gudise Santhosh) > An integral protein...

An integral protein of the inner nuclear membrane localizes to the mitotic spindle in mammalian cells

Buch, Charlotta (author)
Södertörns högskola,Institutionen för livsvetenskaper
Lindberg, Robert (author)
Södertörns högskola,Stockholms universitet,Institutionen för biokemi och biofysik,Södertörn University, Sweden,Institutionen för livsvetenskaper
Figueroa, Ricardo (author)
Södertörns högskola,Stockholms universitet,Institutionen för biokemi och biofysik,Södertörn University, Sweden,Institutionen för livsvetenskaper
show more...
Gudise, Santhosh (author)
Södertörns högskola,Institutionen för livsvetenskaper
Onischenko, Evgeny (author)
Hallberg, Einar (author)
Södertörns högskola,Institutionen för livsvetenskaper
show less...
 (creator_code:org_t)
The Company of Biologists, 2009
2009
English.
In: Journal of Cell Science. - : The Company of Biologists. - 0021-9533 .- 1477-9137. ; 122:12, s. 2100-2107
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • Here, we characterize a transmembrane protein of the nuclear envelope that we name spindle-associated membrane protein 1 (Samp1). The protein is conserved in metazoa and fission yeast and is homologous to Net5 in rat and Ima1 in Schizosaccharomyces pombe. We show that, in human cells, the protein is a membrane-spanning polypeptide with an apparent molecular mass of 43 kDa. This is consistent with a predicted polypeptide of 392 amino acids that has five transmembrane segments and its C-terminus exposed to the nucleoplasm. During interphase, Samp1 was specifically distributed in the inner nuclear membrane. Post-transcriptional silencing of Samp1 expression resulted in separation of centrosomes from the nuclear envelope, indicating that it is functionally connected to the cytoskeleton. At the onset of mitosis, most of the protein dispersed out into the ER, as expected. However, during mitosis, a significant fraction of the protein specifically localized to the polar regions of the mitotic spindle. We demonstrate for the first time, in human cells, the existence of a membranous structure overlapping with the mitotic spindle. Interestingly, another integral inner nuclear membrane protein, emerin, was absent from the spindle-associated membranes. Thus, Samp1 defines a specific membrane domain associated with the mitotic spindle.

Subject headings

NATURVETENSKAP  -- Biologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences (hsv//eng)
NATURVETENSKAP  -- Biologi -- Cellbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)

Keyword

Inner nuclear membrane
Transmembrane proteins
Mitotic spindle
Mitosis
Cancer

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

To the university's database

Search outside SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view