Search: WFRF:(Andersson Anna Maria)
> (2005-2009) >
Elevated Peripheral...
-
Johansson, CatharinaKarolinska Institutet
(author)
Elevated Peripheral Allergen-Specific T Cell Response Is Crucial for a Positive Atopy Patch Test Reaction
- Article/chapterEnglish2009
Publisher, publication year, extent ...
-
2009-04-02
-
S. Karger AG,2009
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:su-60077
-
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-60077URI
-
https://doi.org/10.1159/000210380DOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:118788912URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
authorCount :7
-
Background: Atopic eczema is a chronic inflammatory skin disease in which several subgroups of cases can be identified. Atopy patch testing (APT) reveals allergen sensitization also in atopic eczema patients devoid of detectable allergen-specific IgE, suggesting the importance of factors other than IgE in the reaction. Here we investigate the relationship between APT reactions and allergen-specific peripheral IgE and T cell reactivity in atopic eczema patients. Methods: Adult patients with atopic eczema (n = 64) and healthy controls (n = 24) were analyzed for reactivity to Malassezia sympodialis extract by APT, measurement of specific plasma IgE and in vitro determination of the frequency of allergen-reactive peripheral blood mononuclear cells producing interleukin-4 and interleukin-5 using the ELISpot method. Results: When combining the results of the APT, IgE measurements and the ELISpot analyses, reactivity to M. sympodialis was found in a majority of the atopic eczema patients (69%), whereas the healthy controls were negative throughout. T cell reactivity to M. sympodialis, manifested by production of both interleukins 4 and 5, was highly predictive for a positive APT reaction and displayed a strongly positive correlation with the APT score. In contrast, the allergen-specific IgE levels did not predict the APT outcome, and no correlation could be found between the IgE levels and the APT score. Conclusion: Peripheral allergen-specific T helper 2 cell-mediated reactivity appears to be required for a positive APT reaction to M. sympodialis. The diagnostic potential of measuring peripheral allergen-specific T cell responses should be considered in atopic eczema.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Ahlborg, NiklasStockholms universitet,Avdelningen för immunologi
(author)
-
Andersson, AnnaKarolinska Institutet
(author)
-
Lundeberg, Lena
(author)
-
Karlsson, Maria A.
(author)
-
Scheynius, Annika
(author)
-
Linder, Maria TengvallKarolinska Institutet
(author)
-
Karolinska InstitutetAvdelningen för immunologi
(creator_code:org_t)
Related titles
-
In:International Archives of Allergy and Immunology: S. Karger AG150:1, s. 51-581018-24381423-0097
Internet link
Find in a library
To the university's database