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A dynamic c termina...
A dynamic c terminal segment in the mycobacterium tuberculosis mn/fe r2lox protein can adopt a helical structure with possible functional consequences
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- Andersson, Charlotta S., 1979- (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Berthold, Catrine L. (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Högbom, Martin (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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(creator_code:org_t)
- 2012-09-13
- 2012
- English.
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In: Chemistry and Biodiversity. - : Wiley. - 1612-1872 .- 1612-1880. ; 9:9, s. 1981-1988
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Abstract
Subject headings
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- Mycobacterium tuberculosis R2-like ligand-binding oxidase (MtR2lox) belongs to a recently discovered group of proteins that are homologous to the ribonucleotide reductase R2 proteins. MtR2lox carries a heterodinuclear Mn/Fe cofactor and, unlike R2 proteins, a large ligand-binding cavity. A unique tyrosine-valine cross link is also found in the vicinity of the active site. To date, all known structures of R2 and R2lox proteins show a disordered C-terminal segment. Here, we present two new crystal forms of MtR2lox, revealing an ordered helical C-terminal. The ability of alternating between an ordered and disordered state agrees well with bioinformatic analysis of the protein sequence. Interestingly, ordering of the C-terminal helix shields a large positively charged patch on the protein surface, potentially used for interaction with other cellular components. We hypothesize that the dynamic C-terminal segment may be involved in control of protein function in vivo.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Keyword
- Protein folding
- Metalloproteins
- Structural dynamics
- Ribonucleotide reductases
- Oxidases
- X-Ray crystallography
Publication and Content Type
- ref (subject category)
- art (subject category)
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