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Anionic Lipid Bindi...
Anionic Lipid Binding to the Foreign Protein MGS Provides a Tight Coupling between Phospholipid Synthesis and Protein Overexpression in Escherichia coli
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- Ariöz, Candan, 1983- (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Ye, Weihua (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- al Bakali, Amin (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Ge, Changrong (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Liebau, Jobst (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Götzke, Hansjörg (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Barth, Andreas (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Wieslander, Åke (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Mäler, Lena (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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(creator_code:org_t)
- 2013-08-05
- 2013
- English.
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In: Biochemistry. - : American Chemical Society (ACS). - 0006-2960 .- 1520-4995. ; 52:33, s. 5533-5544
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Certain membrane proteins involved in lipid synthesis can induce formation of new intracellular membranes in Escherichia coli, i.e., intracellular vesicles. Among those, the foreign monotopic glycosyltransferase MGS from Acholeplasma laidlawii triggers such massive lipid synthesis when overexpressed. To examine the mechanism behind the increased lipid synthesis, we investigated the lipid binding properties of MGS in vivo together with the correlation between lipid synthesis and MGS overexpression levels. A good correlation between produced lipid quantities and overexpressed MGS protein was observed when standard LB medium was supplemented with four different lipid precursors that have significant roles in the lipid biosynthesis pathway. Interestingly, this correlation was highest concerning anionic lipid production and at the same time dependent on the selective binding of anionic lipid molecules by MGS. A selective interaction with anionic lipids was also observed in vitro by P-31 NMR binding studies using bicelles prepared with E. coli lipids. The results clearly demonstrate that the discriminative withdrawal of anionic lipids, especially phosphatidylglycerol, from the membrane through MGS binding triggers an in vivo signal for cells to create a feed-forward stimulation of lipid synthesis in E. coil. By this mechanism, cells can produce more membrane surface in order to accommodate excessively produced MGS molecules, which results in an interdependent cycle of lipid and MGS protein synthesis.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- Biochemistry
- biokemi
- Biophysics
- biofysik
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Ariöz, Candan, 1 ...
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Ye, Weihua
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al Bakali, Amin
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Ge, Changrong
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Liebau, Jobst
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Götzke, Hansjörg
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show more...
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Barth, Andreas
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Wieslander, Åke
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Mäler, Lena
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
- Articles in the publication
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Biochemistry
- By the university
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Stockholm University
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Chalmers University of Technology