Detection and Isolation of Sindbis Virus from Mosquitoes Captured During an Outbreak in Sweden, 2013

Bergqvist, JoakimUmeå universitet,Institutionen för klinisk mikrobiologi (författare)

Mosquito-borne alphaviruses have the potential to cause large outbreaks throughout the world. Here we investigated the causative agent of an unexpected Sindbis virus (SINV) outbreak during August-September, 2013, in a previously nonendemic region of Sweden. Mosquitoes were collected using carbon dioxide-baited CDC traps at locations close to human cases. The mosquitoes were initially screened as large pools by SINV-specific quantitative RT-PCR, and the SINV-positive mosquitoes were species determined by single-nucleotide polymorphism (SNP) analysis, followed by sequencing the barcoding region of the cytochrome oxidase I gene. The proportion of the collected mosquitoes was determined by a metabarcoding strategy. By using novel strategies for PCR screening and genetic typing, a new SINV strain, Lovanger, was isolated from a pool of 1600 mosquitoes composed of Culex, Culiseta, and Aedes mosquitoes as determined by metabarcoding. The SINV-positive mosquito Culiseta morsitans was identified by SNP analysis and sequencing. After whole-genome sequencing and phylogenetic analysis, the SINV Lovanger isolate was shown to be most closely similar to recent Finnish SINV isolates. In conclusion, within a few weeks, we were able to detect and isolate a novel SINV strain and identify the mosquito vector during a sudden SINV outbreak.

Forsman, OscarUmeå universitet,Institutionen för klinisk mikrobiologi (författare)
Larsson, PärUmeå universitet,Institutionen för medicinsk biovetenskap(Swepub:umu)pala0006 (författare)
Näslund, JonasUmeå universitet,Institutionen för klinisk mikrobiologi(Swepub:umu)najo7301 (författare)
Lilja, Tobias (författare)
Engdahl, CeciliaUmeå universitet,Kemiska institutionen(Swepub:umu)ceen0001 (författare)
Lindström, Anders (författare)
Gylfe, ÅsaUmeå universitet,Institutionen för klinisk mikrobiologi(Swepub:umu)asagye91 (författare)
Ahlm, ClasUmeå universitet,Institutionen för klinisk mikrobiologi,Arcum(Swepub:umu)clah0001 (författare)
Evander, MagnusUmeå universitet,Institutionen för klinisk mikrobiologi,Arktiskt centrum vid Umeå universitet (Arcum),Arcum(Swepub:umu)maev0001 (författare)
Bucht, Göran[ 1 ] CBRN Def & Secur, Swedish Def Res Agcy, SE-90182 Umea, Sweden (författare)
Umeå universitetInstitutionen för klinisk mikrobiologi (creator_code:org_t)

Ingår i:Vector Borne and Zoonotic Diseases: Mary Ann Liebert Inc15:2, s. 133-1401530-36671557-7759

Av författaren/redakt...: Bergqvist, Joaki ...; Forsman, Oscar; Larsson, Pär; Näslund, Jonas; Lilja, Tobias; Engdahl, Cecilia; visa fler...; Lindström, Ander ...; Gylfe, Åsa; Ahlm, Clas; Evander, Magnus; Bucht, Göran; visa färre...

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