Search: (L773:0027 8424 OR L773:1091 6490) lar1:(su) pers:(Brännström Thomas) >
Structural and kine...
Structural and kinetic analysis of protein-aggregate strains in vivo using binary epitope mapping
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- Bergh, Johan, 1983- (author)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Zetterström, Per (author)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Andersen, Peter M. (author)
- Umeå universitet,Klinisk neurovetenskap
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- Brännström, Thomas (author)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Graffmo, Karin Sixtensdotter, 1960- (author)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Jonsson, P. Andreas (author)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Lang, Lisa (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Danielsson, Jens (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Oliveberg, Mikael (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Marklund, Stefan (author)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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(creator_code:org_t)
- 2015-03-23
- 2015
- English.
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In: Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 112:14, s. 4489-4494
- Related links:
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https://www.pnas.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Despite considerable progress in uncovering the molecular details of protein aggregation in vitro, the cause and mechanism of protein-aggregation disease remain poorly understood. One reason is that the amount of pathological aggregates in neural tissue is exceedingly low, precluding examination by conventional approaches. We present here a method for determination of the structure and quantity of aggregates in small tissue samples, circumventing the above problem. The method is based on binary epitope mapping using anti-peptide antibodies. We assessed the usefulness and versatility of the method in mice modeling the neurodegenerative disease amyotrophic lateral sclerosis, which accumulate intracellular aggregates of superoxide dismutase-1. Two strains of aggregates were identified with different structural architectures, molecular properties, and growth kinetics. Both were different from superoxide dismutase-1 aggregates generated in vitro under a variety of conditions. The strains, which seem kinetically under fragmentation control, are associated with different disease progressions, complying with and adding detail to the growing evidence that seeding, infectivity, and strain dependence are unifying principles of neurodegenerative disease.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Medicinsk biovetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Medical Bioscience (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- protein aggregation
- neurodegeneration
- strain
- amyotrophic lateral sclerosis
- transgenic mice
Publication and Content Type
- ref (subject category)
- art (subject category)
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Bergh, Johan, 19 ...
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Zetterström, Per
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Andersen, Peter ...
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Brännström, Thom ...
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Graffmo, Karin S ...
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Jonsson, P. Andr ...
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Lang, Lisa
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Danielsson, Jens
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Oliveberg, Mikae ...
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Marklund, Stefan
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Pharmacology and ...
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Veterinary Scien ...
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and Medical Bioscien ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
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Proceedings of t ...
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Umeå University
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Stockholm University