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- Turunen, SiruDepartment of Applied Physics, University of Eastern Finland, Kuopio, Finland (författare)
The effect of collagen degradation on chondrocyte volume and morphology in bovine articular cartilage following a hypotonic challenge
- Artikel/kapitelEngelska2013
Förlag, utgivningsår, omfång ...
- 2012-06-19
- Springer,2013
- printrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:umu-103881
- https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-103881URI
- https://doi.org/10.1007/s10237-012-0409-4DOI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
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- SwePubSwePub
Klassifikation
Anmärkningar
- Collagen degradation is one of the early signs of osteoarthritis. It is not known how collagen degradation affects chondrocyte volume and morphology. Thus, the aim of this study was to investigate the effect of enzymatically induced collagen degradation on cell volume and shape changes in articular cartilage after a hypotonic challenge. Confocal laser scanning microscopy was used for imaging superficial zone chondrocytes in intact and degraded cartilage exposed to a hypotonic challenge. Fourier transform infrared microspectroscopy, polarized light microscopy, and mechanical testing were used to quantify differences in proteoglycan and collagen content, collagen orientation, and biomechanical properties, respectively, between the intact and degraded cartilage. Collagen content decreased and collagen orientation angle increased significantly (p < 0.05) in the superficial zone cartilage after collagenase treatment, and the instantaneous modulus of the samples was reduced significantly (p < 0.05). Normalized cell volume and height 20 min after the osmotic challenge (with respect to the original volume and height) were significantly (p < 0.001 and p < 0.01, respectively) larger in the intact compared to the degraded cartilage. These findings suggest that the mechanical environment of chondrocytes, specifically collagen content and orientation, affects cell volume and shape changes in the superficial zone articular cartilage when exposed to osmotic loading. This emphasizes the role of collagen in modulating cartilage mechanobiology in diseased tissue.
Ämnesord och genrebeteckningar
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
- NATURVETENSKAP Biologi Biofysik hsv//swe
- NATURAL SCIENCES Biological Sciences Biophysics hsv//eng
- articular cartilage
- mechanobiology. microscopy
- collagen
- chondrocyte
- enzymatic treatment
- osmotic challenge
- biomechanics
- biomekanik
- cell research
- cellforskning
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Lammi, Mikko,1961-Department of Biomedicine, University of Eastern Finland, Kuopio, Finland; Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group(Swepub:umu)mila0077 (författare)
- Saarakkala, SimoDepartment of Diagnostic Radiology, University of Oulu, Oulu, Finland; Oulu University Hospital, Oulu, Finland (författare)
- Han, Sang-KuyHuman Performance Laboratory, Faculty of Kinesiology, University of Calgary, Calgary, Canada; Mechanical and Manufacturing Engineering, Schulich School of Engineering, University of Calgary, Calgary, Canada; Fischell Department of Bioengineering, Clark School of Engineering, University of Maryland, Maryland, USA (författare)
- Herzog, WalterHuman Performance Laboratory, Faculty of Kinesiology, University of Calgary, Calgary, Canada; Mechanical and Manufacturing Engineering, Schulich School of Engineering, University of Calgary, Calgary, Canada (författare)
- Tanska, PetriDepartment of Applied Physics, University of Eastern Finland, Kuopio, Finland (författare)
- Korhonen, RamiDepartment of Applied Physics, University of Eastern Finland, Kuopio, Finland (författare)
- Department of Applied Physics, University of Eastern Finland, Kuopio, FinlandDepartment of Biomedicine, University of Eastern Finland, Kuopio, Finland; Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Biomechanics and Modeling in Mechanobiology: Springer12:3, s. 417-4291617-79591617-7940
Internetlänk
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- Biomechanics and Modeling in Mechanobiology (Sök värdpublikationen i LIBRIS)
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