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Comparative analysi...
Comparative analysis of gene expression profiles between primary knee osteoarthritis and an osteoarthritis endemic to Northwestern China, Kashin-Beck disease.
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- Duan, Chen (author)
- Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China
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- Guo, Xiong (author)
- Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China
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- Zhang, Xiao-Dong (author)
- First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China
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- Yu, Han-Jie (author)
- Northwest University, Xi'an, Shaanxi, China
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- Yan, Hua (author)
- Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China
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- Gao, Ying (author)
- Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China
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- Ma, Wei-Juan (author)
- Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China
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- Gao, Zong-Qiang (author)
- Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China
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- Xu, Peng (author)
- Xi'an Red Cross Hospital, Xi'an, Shaanxi, China
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- Lammi, Mikko, 1961- (author)
- University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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(creator_code:org_t)
- 2010-02-25
- 2010
- English.
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In: Arthritis and Rheumatism. - : John Wiley & Sons. - 0004-3591 .- 1529-0131. ; 62:3, s. 771-780
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Abstract
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- OBJECTIVE: To investigate the differences in gene expression profiles of adult articular cartilage from patients with Kashin-Beck disease (KBD) versus those with primary knee osteoarthritis (OA).METHODS: The messenger RNA expression profiles of articular cartilage from patients with KBD, diagnosed according to the clinical criteria for KBD in China, were compared with those of cartilage from patients with OA, diagnosed according to the Western Ontario and McMaster Universities OA Index. Total RNA was isolated separately from 4 pairs of the KBD and OA cartilage samples, and the expression profiles were evaluated by Agilent 4x44k Whole Human Genome density oligonucleotide microarray analysis. The microarray data for selected transcripts were confirmed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) amplification.RESULTS: For 1.2 x 10(4) transcripts, corresponding to 58.4% of the expressed transcripts, 2-fold changes in differential expression were revealed. Expression levels higher in KBD than in OA samples were observed in a mean + or - SD 6,439 + or - 1,041 (14.6 + or - 2.4%) of the transcripts, and expression levels were lower in KBD than in OA samples in 6,147 + or - 1,222 (14.2 + or - 2.8%) of the transcripts. After application of the selection criteria, 1.85% of the differentially expressed genes (P < 0.001 between groups) were detected. These included 233 genes, of which 195 (0.4%) were expressed at higher levels and 38 (0.08%) were expressed at lower levels in KBD than in OA cartilage. Comparisons of the quantitative RT-PCR data supported the validity of our microarray data.CONCLUSION: Differences between KBD and OA cartilage exhibited a similar pattern among all 4 of the pairs examined, indicating the presence of disease mechanisms, mainly chondrocyte matrix metabolism, cartilage degeneration, and apoptosis induction pathways, which contribute to cartilage destruction in KBD.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Ortopedi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Orthopaedics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Keyword
- Kashin-Beck disease
- articular cartilage
- gene expression profiling
- cell research
- cellforskning
- genetik
- Genetics
- ortopedi
- Orthopaedics
- patologi
- Pathology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Duan, Chen
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Guo, Xiong
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Zhang, Xiao-Dong
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Yu, Han-Jie
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Yan, Hua
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Gao, Ying
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Ma, Wei-Juan
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Gao, Zong-Qiang
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Xu, Peng
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Lammi, Mikko, 19 ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Orthopaedics
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
- Articles in the publication
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Arthritis and Rh ...
- By the university
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Umeå University