Identification of vitamin C transporters in the human airways: a cross-sectional in vivo study

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: id:"swepub:oai:DiVA.org:umu-106157" > Identification of v...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Larsson, NirinaUmeå universitet,Lungmedicin (author)

Identification of vitamin C transporters in the human airways : a cross-sectional in vivo study

Article/chapterEnglish2015

Publisher, publication year, extent ...

2015-04-08
BMJ,2015
electronicrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:umu-106157
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-106157URI
https://doi.org/10.1136/bmjopen-2014-006979DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Objectives: Vitamin C is an important low-molecular weight antioxidant at the air-lung interface. Despite its critical role as a sacrificial antioxidant, little is known about its transport into the respiratory tract lining fluid (RTLF), or the underlying airway epithelial cells. While several vitamin C transporters have been identified, such as sodium-ascorbate cotransporters (SVCT1/2) and glucose transporters (GLUTs), the latter transporting dehydroascorbate, knowledge of their protein distribution within the human lung is limited, in the case of GLUTs or unknown for SVCTs.Setting and participants: Protein expression of vitamin C transporters (SVCT1/2 and GLUT1-4) was examined by immunohistochemistry in endobronchial biopsies, and by FACS in airway leucocytes from lavage fluid, obtained from 32 volunteers; 16 healthy and 16 mild asthmatic subjects. In addition, antioxidant concentrations were determined in RTLF. The study was performed at one Swedish centre.Primary and secondary outcome measures: The primary outcome measure was to establish the location of vitamin C transporters in the human airways. As secondary outcome measures, RTLF vitamin C concentration was measured and related to transporter expression, as well as bronchial epithelial inflammatory and goblet cells numbers.Results: Positive staining was identified for SVCT1 and 2 in the vascular endothelium. SVCT2 and GLUT2 were present in the apical bronchial epithelium, where SVCT2 staining was predominately localised to goblet cells and inversely related to RTLF vitamin C concentrations.Conclusions: This experimental study is the first to demonstrate protein expression of GLUT2 and SVCT2 in the human bronchial epithelium. A negative correlation between SVCT2-positive goblet cells and bronchial RTLF vitamin C concentrations suggests a possible role for goblet cells in regulating the extracellular vitamin C pool.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

Rankin, Gregory D.Umeå universitet,Lungmedicin(Swepub:umu)grdo0001 (author)
Bicer, Elif M. (author)
Roos-Engstrand, EsterUmeå universitet,Lungmedicin(Swepub:umu)esrrnd01 (author)
Pourazar, JamshidUmeå universitet,Lungmedicin(Swepub:umu)japo0001 (author)
Blomberg, AndersUmeå universitet,Lungmedicin(Swepub:umu)anbl0001 (author)
Mudway, Ian S. (author)
Behndig, Annelie F.Umeå universitet,Lungmedicin(Swepub:umu)anbe0145 (author)
Umeå universitetLungmedicin (creator_code:org_t)

Related titles

In:BMJ Open: BMJ5:42044-6055

Internet link

Find in a library

BMJ Open (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Larsson, Nirina; Rankin, Gregory ...; Bicer, Elif M.; Roos-Engstrand, ...; Pourazar, Jamshi ...; Blomberg, Anders; show more...; Mudway, Ian S.; Behndig, Annelie ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Respiratory Medi ...

Articles in the publication: BMJ Open

By the university: Umeå University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se