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Larsson, NirinaUmeå universitet,Lungmedicin
(author)
Identification of vitamin C transporters in the human airways : a cross-sectional in vivo study
- Article/chapterEnglish2015
Publisher, publication year, extent ...
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2015-04-08
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BMJ,2015
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electronicrdacarrier
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LIBRIS-ID:oai:DiVA.org:umu-106157
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-106157URI
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https://doi.org/10.1136/bmjopen-2014-006979DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Objectives: Vitamin C is an important low-molecular weight antioxidant at the air-lung interface. Despite its critical role as a sacrificial antioxidant, little is known about its transport into the respiratory tract lining fluid (RTLF), or the underlying airway epithelial cells. While several vitamin C transporters have been identified, such as sodium-ascorbate cotransporters (SVCT1/2) and glucose transporters (GLUTs), the latter transporting dehydroascorbate, knowledge of their protein distribution within the human lung is limited, in the case of GLUTs or unknown for SVCTs.Setting and participants: Protein expression of vitamin C transporters (SVCT1/2 and GLUT1-4) was examined by immunohistochemistry in endobronchial biopsies, and by FACS in airway leucocytes from lavage fluid, obtained from 32 volunteers; 16 healthy and 16 mild asthmatic subjects. In addition, antioxidant concentrations were determined in RTLF. The study was performed at one Swedish centre.Primary and secondary outcome measures: The primary outcome measure was to establish the location of vitamin C transporters in the human airways. As secondary outcome measures, RTLF vitamin C concentration was measured and related to transporter expression, as well as bronchial epithelial inflammatory and goblet cells numbers.Results: Positive staining was identified for SVCT1 and 2 in the vascular endothelium. SVCT2 and GLUT2 were present in the apical bronchial epithelium, where SVCT2 staining was predominately localised to goblet cells and inversely related to RTLF vitamin C concentrations.Conclusions: This experimental study is the first to demonstrate protein expression of GLUT2 and SVCT2 in the human bronchial epithelium. A negative correlation between SVCT2-positive goblet cells and bronchial RTLF vitamin C concentrations suggests a possible role for goblet cells in regulating the extracellular vitamin C pool.
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Rankin, Gregory D.Umeå universitet,Lungmedicin(Swepub:umu)grdo0001
(author)
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Bicer, Elif M.
(author)
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Roos-Engstrand, EsterUmeå universitet,Lungmedicin(Swepub:umu)esrrnd01
(author)
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Pourazar, JamshidUmeå universitet,Lungmedicin(Swepub:umu)japo0001
(author)
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Blomberg, AndersUmeå universitet,Lungmedicin(Swepub:umu)anbl0001
(author)
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Mudway, Ian S.
(author)
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Behndig, Annelie F.Umeå universitet,Lungmedicin(Swepub:umu)anbe0145
(author)
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Umeå universitetLungmedicin
(creator_code:org_t)
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In:BMJ Open: BMJ5:42044-6055
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