Recombinant human type II collagen as a material for cartilage tissue engineering.

Pulkkinen, HerttaInstitute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland; Department of Orthopaedics and Traumatology, Jyväskylä Central Hospital, Jyväskylä, Finland (författare)

PURPOSE: Collagen type II is the major component of cartilage and would be an optimal scaffold material for reconstruction of injured cartilage tissue. In this study, the feasibility of recombinant human type II collagen gel as a 3-dimensional culture system for bovine chondrocytes was evaluated in vitro.METHODS: Bovine chondrocytes (4x106 cells) were seeded within collagen gels and cultivated for up to 4 weeks. The gels were investigated with confocal microscopy, histology, and biochemical assays.RESULTS: Confocal microscopy revealed that the cells maintained their viability during the entire cultivation period. The chondrocytes were evenly distributed inside the gels, and the number of cells and the amount of the extracellular matrix increased during cultivation. The chondrocytes maintained their round phenotype during the 4-week cultivation period. The glycosaminoglycan levels of the tissue increased during the experiment. The relative levels of aggrecan and type II collagen mRNA measured with realtime polymerase chain reaction (PCR) showed an increase at 1 week.CONCLUSION: Our results imply that recombinant human type II collagen is a promising biomaterial for cartilage tissue engineering, allowing homogeneous distribution in the gel and biosynthesis of extracellular matrix components.

Tiitu, VirpiInstitute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland; Department of Orthopaedics and Traumatology, Jyväskylä Central Hospital, Jyväskylä, Finland (författare)
Valonen, PiiaInstitute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland; Department of Orthopaedics and Traumatology, Jyväskylä Central Hospital, Jyväskylä, Finland (författare)
Hämäläinen, Eija-RiittaBioprocess Engineering Laboratory, University of Oulu, Oulu, Finland (författare)
Lammi, Mikko,1961-Institute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland; Department of Biosciences, Applied Biotechnology, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group(Swepub:umu)mila0077 (författare)
Kiviranta, IlkkaDepartment of Orthopaedics and Traumatology, Jyväskylä Central Hospital, Jyväskylä, Finland; Department of Orthopaedics and Traumatology, Helsinki University Hospital, Helsinki, Finland (författare)
Institute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland; Department of Orthopaedics and Traumatology, Jyväskylä Central Hospital, Jyväskylä, FinlandBioprocess Engineering Laboratory, University of Oulu, Oulu, Finland (creator_code:org_t)

Ingår i:International Journal of Artificial Organs: Wichtig Editore Srl31:11, s. 960-9690391-39881724-6040

Av författaren/redakt...: Pulkkinen, Hertt ...; Tiitu, Virpi; Valonen, Piia; Hämäläinen, Eija ...; Lammi, Mikko, 19 ...; Kiviranta, Ilkka

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