Human articular cartilage proteoglycans are not undersulfated in osteoarthritis.

Qu, Cheng-Juan,1967-Institute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group (author)

Chondroitin sulfate is the major constituent of cartilage. Inadequate sulfate availability results in the production of undersulfated proteoglycans. In osteoarthritis, there is a net loss of articular cartilage proteoglycans. Theoretically, it is possible that during the progress of disease undersulfated glycosaminoglycans are synthesized producing proteoglycans with poorer biological properties. In this study, we tested whether in early human osteoarthritic articular cartilage (Mankin's score of 2 and 3) or more advanced disease (Mankin's score over 3), there are proteoglycans that contain a higher relative amount of nonsulfated chondroitin disaccharide isomer in their chondroitin sulfate chains by analyzing the molar ratios of chondroitin sulfate disaccharide isoforms with fluorophore-assisted carbohydrate electrophoresis. Our results indicated that the nonsulfated disaccharide of chondroitin sulfate formed in average only 1-2% of the total chondroitin sulfate. More important, the molar ratio of nonsulfated disaccharide did not appear to be increased in the osteoarthritic articular cartilage. We conclude that undersulfation of articular cartilage proteoglycans is not present in the human osteoarthritic joint.

Rieppo, JarnoInstitute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland (author)
Hyttinen, MikaInstitute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland (author)
Lammi, Mikko,1961-Institute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group(Swepub:umu)mila0077 (author)
Kiviranta, IlkkaDepartment of Surgery, Jyväskylä Central Hospital, Jyväskylä, Finland (author)
Kurkijärvi, JattaDepartment of Physics, University of Kuopio, Kuopio, Finland (author)
Jurvelin, JukkaDepartment of Physics, University of Kuopio, Kuopio, Finland; Department of Clinical Physiology and Nuclear Medicine, Kuopio University Hospital, Kuopio, Finland (author)
Töyräs, JuhaDepartment of Clinical Neurophysiology, Kuopio University Hospital, Kuopio, Finland (author)
Institute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, FinlandChondrogenic and Osteogenic Differentiation Group (creator_code:org_t)

In:Connective Tissue Research: Informa Healthcare48:1, s. 27-330300-82071607-8438

By the author/editor: Qu, Cheng-Juan, ...; Rieppo, Jarno; Hyttinen, Mika; Lammi, Mikko, 19 ...; Kiviranta, Ilkka; Kurkijärvi, Jatt ...; show more...; Jurvelin, Jukka; Töyräs, Juha; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Orthopaedics

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