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Geldanamycin activa...
Geldanamycin activates Hsp70 response and attenuates okadaic acid-induced cytotoxicity in human retinal pigment epithelial cells.
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- Kaarniranta, Kai (författare)
- Department of Ophthalmology, University of Kuopio, Kuopio, Finland; Department of Ophthalmology, Kuopio University Hospital, Kuopio, Finland
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- Ryhänen, Tuomas (författare)
- Department of Ophthalmology, University of Kuopio, Kuopio, Finland
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- Sironen, Reijo (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Suuronen, Tiina (författare)
- Department of Neuroscience and Neurology, University of Kuopio, Kuopio, Finland
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- Elo, Mika (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Karjalainen, Hannu (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Lammi, Mikko, 1961- (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Teräsvirta, Markku (författare)
- Department of Ophthalmology, Kuopio University Hospital, Kuopio, Finland
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- Uusitalo, Hannu (författare)
- Department of Neuroscience and Neurology, University of Kuopio, Kuopio, Finland; Department of Neurology, Kuopio University Hospital, Kuopio, Finland
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- Salminen, Antero (författare)
- Department of Neuroscience and Neurology, University of Kuopio, Kuopio, Finland; Department of Neurology, Kuopio University Hospital, Kuopio, Finland
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(creator_code:org_t)
- Elsevier, 2005
- 2005
- Engelska.
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Ingår i: Brain Research. Molecular Brain Research. - : Elsevier. - 0169-328X .- 1872-6941. ; 137:1-2, s. 126-131
- Relaterad länk:
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http://www.sciencedi...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Reversible protein phosphorylation regulates the biological activities of many human proteins involved in crucial cellular processes, e.g., protein-protein interactions, cell signaling, gene transcription, cell growth, and death. A malfunction of cellular homeostasis in retinal pigment epithelial (RPE) cells is involved in the age-related retinal degeneration. In this study, we examined cytotoxicity in human RPE cells subjected to the protein phosphatase inhibitor, okadaic acid (OA). Moreover, the influence of Hsp90 inhibitor geldanamycin (GA), a benzoquinone ansamycin, in cytoprotection was assessed. Hsp70 protein levels were analyzed by Western blot. Cellular viability was determined by LDH and MTT assays. To study apoptotic cell death, caspase-3 enzyme activity was measured by assaying the cleavage of a fluorescent peptide substrate and Hoechst dye was used to visualize nuclear morphology. OA treatment caused morphological changes and induced cytotoxicity by caspase-3-independent manner in the RPE cells. No evidence of nuclear fragmentation was observed in response to OA. Interestingly, GA treatment accumulated Hsp70 protein and attenuated OA-induced cytotoxicity. This study suggests that Hsp70 and Hsp90 are closely related to cytoprotection of RPE cells in response to protein phosphatase inhibition.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Oftalmologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Ophthalmology (hsv//eng)
Nyckelord
- Opthalmology
- retinal pigment epithelial cells
- geldanamycin
- heat shock protein
- cell viability
- biokemi
- Biochemistry
- cell research
- cellforskning
- oftalmiatrik
- Ophtalmology
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Till lärosätets databas
- Av författaren/redakt...
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Kaarniranta, Kai
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Ryhänen, Tuomas
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Sironen, Reijo
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Suuronen, Tiina
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Elo, Mika
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Karjalainen, Han ...
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visa fler...
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Lammi, Mikko, 19 ...
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Teräsvirta, Mark ...
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Uusitalo, Hannu
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Salminen, Antero
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och Oftalmologi
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