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Undersulfated chond...
Undersulfated chondroitin sulfate does not increase in osteoarthritic cartilage.
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- Lammi, Mikko, 1961- (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Qu, Cheng-Juan, 1967- (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Laasanen, Mikko (författare)
- Department of Applied Physics, University of Kuopio, Kuopio, Finland
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- Saarakkala, Simo (författare)
- Department of Applied Physics, University of Kuopio, Kuopio, Finland
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- Rieppo, Jarno (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Jurvelin, Jukka (författare)
- Department of Applied Physics, University of Kuopio, Kuopio, Finland
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- Töyräs, Juha (författare)
- Department of Applied Physics, University of Kuopio, Kuopio, Finland
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(creator_code:org_t)
- Journal of Rheumatology, 2004
- 2004
- Engelska.
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Ingår i: Journal of Rheumatology. - : Journal of Rheumatology. - 0315-162X .- 1499-2752. ; 31:12, s. 2449-2453
- Relaterad länk:
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http://www.jrheum.or...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- OBJECTIVE: To test whether there is undersulfation of chondroitin sulfate in osteoarthritic bovine articular cartilage to support the hypothesis that sulfate deficiency is involved with the development of osteoarthritis.METHODS: Cartilage samples from bovine patellae (n = 32) were divided into 3 groups based on their osteoarthritic progression, as assessed by modified Mankin score. Uronic acid contents of the samples were determined. Fragmentation of the proteoglycans due to proteolytic processing was estimated with agarose gel electrophoresis. The molar ratios of chondroitin sulfate isoforms in the extracted proteoglycans were determined with fluorophore-assisted carbohydrate electrophoresis.RESULTS: Loss of proteoglycans and accumulation of tissue water was evident in groups II and III, and progressive OA increased heterogeneity of aggrecan population in groups II and III. Importantly, the molar ratio of nonsulfated disaccharide was decreased in the osteoarthritic articular cartilage.CONCLUSION: The structure of chondroitin sulfate in degenerated bovine cartilage did not support the hypothesis that sulfate depletion is present in osteoarthritic joint.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Articular cartilage
- chondrocyte
- proteoglycan
- sulfation
- osteoarthritis
- bovine
- biokemi
- Biochemistry
- cell research
- cellforskning
- reumatologi
- rheumatology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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