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EGF upregulates, wh...
EGF upregulates, whereas TGF-beta downregulates, the hyaluronan synthases Has2 and Has3 in organotypic keratinocyte cultures: correlations with epidermal proliferation and differentiation.
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- Pasonen-Seppänen, Sanna (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Karvinen, Susanna (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Törrönen, Kari (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Hyttinen, Juha (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Jokela, Tiina (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Lammi, Mikko, 1961- (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Tammi, Markku (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Tammi, Raija (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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(creator_code:org_t)
- Nature Publishing Group, 2003
- 2003
- Engelska.
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Ingår i: Journal of Investigative Dermatology. - : Nature Publishing Group. - 0022-202X .- 1523-1747. ; 120:6, s. 1038-1044
- Relaterad länk:
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http://www.nature.co...
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http://www.jidonline...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Hyaluronan, a major extracellular matrix molecule in the vital cell layers of skin epidermis, has been suggested to support proliferation and migration of keratinocytes, during challenges like wounding and inflammation. An organotypic keratinocyte culture originated from continuous rat epidermal keratinocyte cell line was subjected to the proliferative and antiproliferative growth factors epidermal growth factor and transforming growth factor beta, respectively, to study their influence on hyaluronan synthesis and epidermal morphology. Epidermal growth factor induced a 4-fold increase of epidermal hyaluronan concentration. This was associated with upregulation of the hyaluronan synthases Has2 and Has3, and the hyaluronan receptor CD44. 5-Bromo-2'-deoxyuridine labeling, basal cell height, and the thickness of vital epidermis were increased, reflecting the hyperplastic effects of epidermal growth factor. The expression of keratin 10 and the maturation of filaggrin were inhibited, and epidermal permeability barrier became less efficient, indicating compromised terminal differentiation by epidermal growth factor. In contrast, transforming growth factor beta reduced the content of hyaluronan and the mRNA of Has2 and Has3. At the same time, transforming growth factor beta suppressed keratinocyte proliferation and epidermal thickness, but retained intact differentiation. The results suggest that epidermal hyaluronan synthesis, controlled by epidermal growth factor and transforming growth factor beta through changes in the expression of Has2 and Has3, correlates with epidermal proliferation, thickness, and differentiation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Nyckelord
- Skin
- epidermal growth factor
- hyaluronan
- HAS2
- HAS3
- CD44
- filaggrin
- keratin
- cell research
- cellforskning
- Dermatology and Venerology
- dermatologi och venereologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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