Type X collagen, a natural component of mouse articular cartilage: association with growth, aging, and osteoarthritis.

Eerola, IiroDepartment of Medical Biochemistry and Molecular Biology, University of Turku, Turku, Finland (author)

Type X collagen, a natural component of mouse articular cartilage : association with growth, aging, and osteoarthritis.

OBJECTIVE: To perform a systematic study on the production and deposition of type X collagen in developing, aging, and osteoarthritic (OA) mouse articular cartilage.METHODS: Immunohistochemistry was employed to define the distribution of type X collagen and Northern analyses to determine the messenger RNA levels as an indicator of the synthetic activity of the protein.RESULTS: Type X collagen was observed in the epiphyseal and articular cartilage of mouse knee joints throughout development and growth. Type X collagen deposition in the transitional zone of articular cartilage became evident toward cessation of growth, at the age of 2-3 months. The most intense staining for type X collagen was limited to the tidemark, the border between uncalcified and calcified cartilage. Northern analysis confirmed that the type X collagen gene is also transcribed by articular cartilage chondrocytes. Intense immunostaining was observed in the areas of OA lesions, specifically, at sites of osteophyte formation and surface fibrillation. Type X collagen deposition was also seen in degenerating menisci.CONCLUSION: This study demonstrates that type X collagen is a natural component of mouse articular cartilage throughout development, growth, and aging. This finding and the deposition of type X collagen at sites of OA lesions suggest that type X collagen may have a role in providing structural support for articular cartilage.

Salminen, HeliDepartment of Medical Biochemistry and Molecular Biology, University of Turku, Turku, Finland (author)
Lammi, PirkkoDepartment of Anatomy, University of Kuopio, Kuopio, Finland (author)
Lammi, Mikko,1961-Department of Anatomy, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group(Swepub:umu)mila0077 (author)
von der Mark, KlausInstitute of Experimental Medicine, Friedrich Alexander University of Erlangen-Nuremberg, Erlangen, Germany (author)
Vuorio, EeroDepartment of Medical Biochemistry and Molecular Biology, University of Turku, Turku, Finland (author)
Säämänen, Anna-MarjaDepartment of Medical Biochemistry and Molecular Biology, University of Turku, Turku, Finland (author)
Department of Medical Biochemistry and Molecular Biology, University of Turku, Turku, FinlandDepartment of Anatomy, University of Kuopio, Kuopio, Finland (creator_code:org_t)

In:Arthritis and Rheumatism: John Wiley & Sons41:7, s. 1287-12950004-35911529-0131

By the author/editor: Eerola, Iiro; Salminen, Heli; Lammi, Pirkko; Lammi, Mikko, 19 ...; von der Mark, Kl ...; Vuorio, Eero; show more...; Säämänen, Anna-M ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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