Safranin O reduces loss of glycosaminoglycans from bovine articular cartilage during histological specimen preparation.

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Király, KariDepartment of Anatomy, University o Kuopio, Kuopio, Finland (författare)

Safranin O reduces loss of glycosaminoglycans from bovine articular cartilage during histological specimen preparation.

Artikel/kapitelEngelska1996

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Chapman & Hill,1996
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LIBRIS-ID:oai:DiVA.org:umu-108052
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-108052URI

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Språk:engelska
Sammanfattning på:engelska &language:-1_t

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The ability of Safranin O, added to fixation and decalcification solutions, to prevent the escape of glycosaminoglycans (GAGs) from small cartilage tissue blocks during histological processing of cartilage has been studied. GAGs in the fixatives and decalcifying solutions used and those remaining in the 1 mm3 cubes of cartilage were assayed biochemically. The quantity of GAGs remaining in the cartilage cubes were determined from Safranin O-stained sections using videomicroscopy or microspectrophotometry. A quantity (10.6%) of GAGs were lost during a conventional 4% buffered formaldehyde fixation (48 h) and a subsequent decalcification in 10% EDTA (12 days) at 4 degrees C. Roughly one-quarter of the total GAG loss occurred during the 48 h fixation, and three-quarters during the 12 days of decalcification. Inclusion of 4% formaldehyde in the decalcification fluid decreased the loss of GAGs to 6.2%. The presence of 0.5% Safranin O in the fixative reduced this loss to 3.4%. When 0.5% Safranin O was included in the fixative and 4% formaldehyde in the decalcification solution, Safranin O staining of the histological sections increased on average by 13.5%. After fixation in the presence of 0.5% Safranin O, there was no difference in the staining intensities when decalcification was carried out in the presence of either Safranin O or formaldehyde, or both. It took 24 h for Safranin O to penetrate into the deep zone of articular cartilage, warranting a fixation period of at least this long. In conclusion, the addition of Safranin O to the fixative and either Safranin O or formaldehyde in the following decalcification fluid, markedly reduces the loss of GAGs from small articular cartilage explants during histological processing. However, for immunohistochemical studies, Safranin O cannot be included in the processing solutions, because it may interfere.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Ortopedi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Orthopaedics hsv//eng
Articular cartilage
proteoglycans
histology
sample preparation
biokemi
Biochemistry
cell research
cellforskning
ortopedi
Orthopaedics

Biuppslag (personer, institutioner, konferenser, titlar ...)

Lammi, Mikko,1961-Department of Anatomy, University o Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group(Swepub:umu)mila0077 (författare)
Arokoski, JariDepartment of Anatomy, University o Kuopio, Kuopio, Finland (författare)
Lapveteläinen, TuomoDepartment of Anatomy, University o Kuopio, Kuopio, Finland (författare)
Tammi, MarkkuDepartment of Anatomy, University o Kuopio, Kuopio, Finland (författare)
Helminen, HeikkiDepartment of Anatomy, University o Kuopio, Kuopio, Finland (författare)
Kiviranta, Ilkka (författare)
Department of Anatomy, University o Kuopio, Kuopio, FinlandChondrogenic and Osteogenic Differentiation Group (creator_code:org_t)

Sammanhörande titlar

Ingår i:The Histochemical Journal: Chapman & Hill28:2, s. 99-1070018-22141573-6865

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Av författaren/redakt...: Király, Kari; Lammi, Mikko, 19 ...; Arokoski, Jari; Lapveteläinen, T ...; Tammi, Markku; Helminen, Heikki; visa fler...; Kiviranta, Ilkka; visa färre...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Ortopedi

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Av lärosätet: Umeå universitet

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