Quantitative Binding Assay for Measuring Specific IgG Antibodies to Alpha-Gal Using the Neoglycoprotein Gal-alpha-1,3-Gal-beta-1,4-Glcnac-Human Serum Albumin

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Schuyler, Alexander J (author)

Quantitative Binding Assay for Measuring Specific IgG Antibodies to Alpha-Gal Using the Neoglycoprotein Gal-alpha-1,3-Gal-beta-1,4-Glcnac-Human Serum Albumin

Article/chapterEnglish2015

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Elsevier,2015
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LIBRIS-ID:oai:DiVA.org:umu-109957
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-109957URI
https://doi.org/10.1016/j.jaci.2014.12.1548DOI

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Language:English
Summary in:English

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Subject category:vet swepub-contenttype
Subject category:art swepub-publicationtype

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Tick bites are known to induce IgE production to alpha-gal. Elevated alpha-gal IgG1 compared to natural alpha-gal IgG2 production has been reported in alpha-gal IgE+ subjects. We here report further investigation of the relationship between alpha-gal IgG and both reactions to red meat and exposure to ticks. Methods: IgG from serum was absorbed onto recombinant Protein G-Sepharose and incubated with radiolabeled allergen. The radioactivity of bound allergen was measured using a gamma counter. A control curve was generated in parallel to assign unitage. Additional testing of serum immunoglobulins was performed via ImmunoCAP and nephelometry. Results: Alpha-gal IgG was measured in a Northern Sweden cohort and in subjects presenting to allergy clinics in Virginia with delayed reactions to red meat. Alpha-gal IgG was significantly higher in alpha-gal IgE+ subjects versus alpha-gal IgE- subjects, and longitudinal serology in several alpha-gal IgE+ subjects demonstrates parallel alpha-gal IgE and IgG response trends. Among the alpha-gal IgE+ subjects, alpha-gal IgG was higher in those with alpha-gal IgE:total IgE ratios >25%, but was not related to reported severity to red meat. Compared to the alpha-gal IgE- subjects in Virginia, alpha-gal IgG was lower in the group from Northern Sweden, where alpha-gal IgE-mediated hypersensitivity is absent and ticks are rare. Conclusions: Alpha-gal IgG is strongly related to alpha-gal IgE and is significantly lower in prevalence and titer in subjects without tick exposure. The absence of a relationship between alpha-gal IgG and severity of reactions to red meat suggests that the alpha-gal syndrome may not be a suitable candidate for conventional immunotherapy.

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James, Hayley R (author)
Rispens, Theo (author)
Workman, Lisa J (author)
Perzanowski, Matthew S (author)
Rönmark, EvaUmeå universitet,Institutionen för folkhälsa och klinisk medicin,The Olin Unit(Swepub:umu)evra0004 (author)
Commins, Scott P (author)
Platts-Mills, Thomas A E (author)
Umeå universitetInstitutionen för folkhälsa och klinisk medicin (creator_code:org_t)

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In:Journal of Allergy and Clinical Immunology: Elsevier135:2, s. AB188-AB1880091-67491097-6825

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Respiratory Medi ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Immunology in th ...

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By the university: Umeå University

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