Sentinel node lymphocytes : tumour reactive lymphocytes identified intraoperatively for the use in immunotherapy of colon cancer

• The sentinel node is the first lymph node to receive lymphatic drainage from a tumour and is usually the first site of metastases. Today, the sentinel node is used for tumour staging. Here, we focus on its immunological role and investigate lymphocytic function in sentinel nodes, identified intraoperatively by peritumoural dye injection, from 15 patients with colon cancer. Tumour infiltrating lymphocytes, sentinel and nonsentinel lymph node cells and peripheral blood leukocytes were studied by flow cytometry, proliferation assays and interferon-gamma secretion after activation with autologous tumour homogenate. Whereas tumour-infiltrating lymphocytes were nonresponsive in the proliferation assays, lymphocytes from sentinel nodes proliferated dose dependently and secreted interferon-gamma upon stimulation with tumour homogenate. The responses were of varying magnitude and tended to be weaker in metastatic sentinel nodes. Sentinel node lymphocytes represents an enriched source of tumour reactive lymphocytes, and may be useful in future trials of adoptive immunotherapy.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine (hsv//eng)

Nyckelord

Aged

Aged

80 and over

CD4-Positive T-Lymphocytes/*immunology

CD8-Positive T-Lymphocytes/*immunology

Colonic Neoplasms/*immunology/pathology/surgery

Female

Flow Cytometry

Humans

Immunotherapy

Lymph Nodes/immunology

Lymphatic Metastasis

Lymphocyte Activation

Lymphocytes

Tumor-Infiltrating/*immunology

Male

Middle Aged

Neoplasm Staging

Sentinel Lymph Node Biopsy

Survival Rate

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)