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The effect of freez...
The effect of freeze/thaw cycles on reproducibility of metabolic profiling of marine microalgal extracts using direct infusion high-resolution mass spectrometry (HR-MS)
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- Eilertsen, Hans Chr (författare)
- Universitetet i Tromsø
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- Huseby, Siv (författare)
- Universitetet i Tromsø
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- Degerlund, Maria (författare)
- Universitetet i Tromsø
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- Eriksen, Gunilla K. (författare)
- Universitetet i Tromsø
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- Ingebrigtsen, Richard A. (författare)
- Universitetet i Tromsø
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- Hansen, Espen (författare)
- Universitetet i Tromsø
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(creator_code:org_t)
- 2014-10-13
- 2014
- Engelska.
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Ingår i: Molecules. - : MDPI. - 1431-5157 .- 1420-3049. ; 19:10, s. 16373-16380
- Relaterad länk:
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https://doi.org/10.3...
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- During normal sample preparation, storage in freezers and subsequent freeze/thaw cycles are commonly introduced. The effect of freeze/thaw cycles on the metabolic profiling of microalgal extracts using HR-MS was investigated. Methanolic extracts of monocultures of Arctic marine diatoms were analyzed immediately after extraction, after seven days of storage at -78 degrees C (one freeze/thaw cycle), and after additional seven days at -20 degrees C (two freeze/thaw cycles). Repeated direct infusion high-resolution mass spectrometry analysis of microalgae extracts of the same sample showed that reproducibility was ca. 90% when a fresh (unfrozen) sample was analyzed. The overall reproducibility decreased further by ca. 10% after the first freeze/thaw-cycle, and after one more freeze/thaw cycle the reproducibility decreased further by ca. 7%. The decrease in reproducibility after freeze-thaw cycles could be attributed to sample degradation and not to instrument variability.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- metabolic profiling
- metabolomics
- microalgae
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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