An intact light harvesting complex I antenna system is required for complete state transitions in Arabidopsis

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: (id:"swepub:oai:DiVA.org:umu-113724") > An intact light har...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Benson, Samuel L (author)

An intact light harvesting complex I antenna system is required for complete state transitions in Arabidopsis

Article/chapterEnglish2015

Publisher, publication year, extent ...

Nature Publishing Group,2015
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:umu-113724
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-113724URI
https://doi.org/10.1038/NPLANTS.2015.176DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Efficient photosynthesis depends on maintaining balance between the rate of light-driven electron transport occurring in photosystem I (PSI) and photosystem II (PSII), located in the chloroplast thylakoid membranes. Balance is achieved through a process of 'state transitions' that increases energy transfer towards PSI when PSII is overexcited (state II), and towards PSII when PSI is overexcited (state I). This is achieved through redox control of the phosphorylation state of light-harvesting antenna complex II (LHCII). PSI is served by both LHCII and four light-harvesting antenna complex I (LHCI) subunits, Lhca1, 2, 3 and 4. Here we demonstrate that despite unchanged levels of LHCII phosphorylation, absence of specific Lhca subunits reduces state transitions in Arabidopsis. The severest phenotype-observed in a mutant lacking Lhca4 (Delta Lhca4)-displayed a 69% reduction compared with the wild type. Yet, surprisingly, the amounts of the PSI-LHCI-LHCII supercomplex isolated by blue native polyacrylamide gel electrophoresis (BN-PAGE) from digitonin-solubilized thylakoids were similar in the wild type and Delta Lhca mutants. Fluorescence excitation spectroscopy revealed that in the wild type this PSI-LHCI-LHCII supercomplex is supplemented by energy transfer from additional LHCII trimers in state II, whose binding is sensitive to digitonin, and which are absent in Delta Lhca4. The grana margins of the thylakoid membrane were found to be the primary site of interaction between this 'extra' LHCII and the PSI-LHCI-LHCII supercomplex in state II. The results suggest that the LHCI complexes mediate energetic interactions between LHCII and PSI in the intact membrane.

Subject headings and genre

NATURVETENSKAP Biologi Botanik hsv//swe
NATURAL SCIENCES Biological Sciences Botany hsv//eng
LANTBRUKSVETENSKAPER Bioteknologi med applikationer på växter och djur Växtbioteknologi hsv//swe
AGRICULTURAL SCIENCES Agricultural Biotechnology Plant Biotechnology hsv//eng

Added entries (persons, corporate bodies, meetings, titles ...)

Maheswaran, Pratheesh (author)
Ware, Maxwell A (author)
Hunter, C Neil (author)
Horton, Peter (author)
Jansson, Stefan,1959-Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)(Swepub:umu)stja0001 (author)
Ruban, Alexander V (author)
Johnson, Matthew P (author)
Umeå universitetInstitutionen för fysiologisk botanik (creator_code:org_t)

Related titles

In:Nature plants: Nature Publishing Group1:122055-026X

Internet link

Find in a library

Nature plants (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Benson, Samuel L; Maheswaran, Prat ...; Ware, Maxwell A; Hunter, C Neil; Horton, Peter; Jansson, Stefan, ...; show more...; Ruban, Alexander ...; Johnson, Matthew ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Botany

AGRICULTURAL SCIENCES: AGRICULTURAL SCI ...; and Agricultural Bio ...; and Plant Biotechnol ...

Articles in the publication: Nature plants

By the university: Umeå University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se