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Use of two-dimensio...
Use of two-dimensional protein pattern analysis for the characterization of Arabidopsis thaliana mutants.
- Article/chapterEnglish1994
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LIBRIS-ID:oai:DiVA.org:umu-114233
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-114233URI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Times Cited: 80 90
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Total proteins extracted from wild-type plants of Arabidopsis thaliana Heyhn, an etiolated mutant, a de-etiolated mutant and a mutant affected in cotyledon morphology, were analyzed by two-dimensional gel electrophoresis. Computer analysis of two-dimensional gels allowed the characterization of the mutants by a set of proteins showing a differential expression when compared with the wild-type plant grown under the same conditions. The overlap between comparisons of the different mutants with the wild-type allowed the identification of groups of polypeptides which, since their expression is altered in several mutants, might be involved in certain physiological functions. For example, this approach showed a possible involvement of actin in the elongation process. The simultaneous analysis of the two-dimensional protein patterns of different mutants seems, therefore, to be a promising approach to characterize proteins involved in various physiological functions.
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Bellini, C.
(author)
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Caboche, M.
(author)
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In:Planta192:4, s. 557-5660032-09351432-2048
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