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Detection of microm...
Detection of micrometastases by flow cytometry in sentinel lymph nodes from patients with renal tumours
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Hartana, Ciputra Adijaya (author)
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Kinn, Johan (author)
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- Rosenblatt, Robert (author)
- Umeå universitet,Urologi och andrologi
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Anania, Stefan (author)
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Alamdari, Farhood (author)
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Glise, Hans (author)
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- Sherif, Amir (author)
- Umeå universitet,Urologi och andrologi
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- Winqvist, Ola (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2016-09-06
- 2016
- English.
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In: British Journal of Cancer. - : Springer Science and Business Media LLC. - 0007-0920 .- 1532-1827. ; 115:8, s. 957-966
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- BACKGROUND: Stage is an important prognostic factor in renal tumours and dissemination to regional lymph nodes is associated with poor outcomes. Lymph nodes are routinely assessed by immunohistochemistry and microscopic evaluation, a time-consuming process where micrometastases might go undiagnosed. We evaluate an alternative method for detecting metastatic cells in sentinel nodes (SNs) by flow cytometry.METHODS: A total of 15 nodes from 5 patients diagnosed with renal tumours were analysed by flow cytometry. Staining for the intracellular marker cytokeratin 18 (CK18) with the surface markers carbonic anhydrase IX (CA9) and Cadherin 6 were used in flow cytometry analysis. Peripheral blood mononuclear cells (PBMCs) with the addition of known concentrations of cancer cell lines were analysed to investigate the sensitivity of micrometastasis detection.RESULTS: Stability of the assay was marked by low intra-assay variability (coefficient of variance ⩽16%) and low inter-assay variability (R(2)=0.9996-1). Eight nodes in four patients were positive for metastasis; six of them were considered being micrometastatic. These metastases were undetected by routine pathology and the patients were restaged from pN0 to pN1.CONCLUSIONS: Flow cytometry is able to detect micrometastases in lymph nodes of renal tumour patients that were undetected under H&E examination.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
Keyword
- kidney neoplasms
- sentinel lymph node biopsy
- lymphatic metastasis
- flow cytometry
Publication and Content Type
- ref (subject category)
- art (subject category)
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