The two Dps proteins, NpDps2 and NpDps5, are involved in light-induced oxidative stress tolerance in the N-2-fixing cyanobacterium Nostoc punctiforme

Cyanobacteria are photosynthetic prokaryotes that are considered biotechnologically prominent organisms for production of high-value compounds. Cyanobacteria are subject to high-light intensities, which is a challenge that needs to be addressed in design of efficient bio-engineered photosynthetic organisms. Dps proteins are members of the ferritin superfamily and are omnipresent in prokaryotes. They play a major role in oxidative stress protection and iron homeostasis. The filamentous, heterocyst-forming Nostoc punctiforme, has five Dps proteins. In this study we elucidated the role of these Dps proteins in acclimation to high light intensity, the gene loci organization and the transcriptional regulation of all five dps genes in N. punctiforme was revealed, and dps-deletion mutant strains were used in physiological characterization. Two mutants defective in Dps2 and Dps5 activity displayed a reduced fitness under increased illumination, as well as a differential Photosystem (PS) stoichiometry, with an elevated Photosystem II to Photosystem I ratio in the dps5 deletion strain. This work establishes a Dps-mediated link between light tolerance, H2O2 detoxification, and iron homeostasis, and provides further evidence on the non-redundant role of multiple Dps proteins in this multicellular cyanobacterium.

Li, XinUppsala universitet,Molekylär biomimetik(Swepub:uu)xinli292 (författare)
Vavitsas, KonstantinosUppsala universitet,Molekylär biomimetik,Univ Copenhagen, Dept Plant & Environm Sci, CPSC, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark (författare)
Dzhygyr, IevgenUppsala universitet,Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Chemistry – Ångström Laboratory, Uppsala University, Uppsala, Sweden,Molekylär biomimetik,Umea Univ, Dept Mol Biol, 6K & 6L Sjukhusomradet, S-90187 Umea, Sweden(Swepub:umu)eudz0001 (författare)
Sandh, GustafUppsala universitet,Stockholms universitet,Institutionen för ekologi, miljö och botanik,Uppsala University, Sweden,Molekylär biomimetik,Stockholm Univ, Dept Ecol Environm & Plant Sci, SciLifeLab, Box 1031, S-17121 Solna, Sweden(Swepub:uu)gussa922 (författare)
Magnuson, AnnUppsala universitet,Molekylär biomimetik(Swepub:uu)anmag178 (författare)
Stensjö, KarinUppsala universitet,Molekylär biomimetik(Swepub:uu)kafor020 (författare)
Uppsala universitetMolekylär biomimetik (creator_code:org_t)

Ingår i:Biochimica et Biophysica Acta - Bioenergetics: Elsevier1857:11, s. 1766-17760005-27281879-2650

Av författaren/redakt...: Moparthi, Vamsi; Li, Xin; Vavitsas, Konsta ...; Dzhygyr, Ievgen; Sandh, Gustaf; Magnuson, Ann; visa fler...; Stensjö, Karin; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.