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Site-directed mutag...
Site-directed mutagenesis and its application in studying the interactions of T3S components
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- Francis, Matthew S (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Umeå Centre for Microbial Research (UCMR),Francis
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- Amer, Ayad A A (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Umeå Centre for Microbial Research (UCMR),Helmholtz Centre for Infection Research, Braunschweig, Germany,Francis
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- Milton, Debra (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Umeå Centre for Microbial Research (UCMR),Department of Biological and Environmental Sciences, Troy University, Troy, AL, USA,Milton
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- Costa, Tiago R D (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Umeå Centre for Microbial Research (UCMR),Institute of Structural and Molecular Biology, University College London and Birkbeck, London, UK,Francis
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(creator_code:org_t)
- 2016-11-12
- 2017
- Engelska.
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Ingår i: Type 3 secretion systems. - New York, NY : Humana Press. - 9781493966479 - 9781493966493 ; , s. 11-31
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Type III secretion systems are a prolific virulence determinant among Gram-negative bacteria. They are used to paralyze the host cell, which enables bacterial pathogens to establish often fatal infections—unless an effective therapeutic intervention is available. However, as a result of a catastrophic rise in infectious bacteria resistant to conventional antibiotics, these bacteria are again a leading cause of worldwide mortality. Hence, this report describes a pDM4-based site-directed mutagenesis strategy that is assisting in our foremost objective to better understand the fundamental workings of the T3SS, using Yersinia as a model pathogenic bacterium. Examples are given that clearly document how pDM4-mediated site-directed mutagenesis has been used to establish clean point mutations and in-frame deletion mutations that have been instrumental in identifying and understanding the molecular interactions between components of the Yersinia type III secretion system.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Site-directed mutagenesis
- Type III secretion systems
- Suicide vector pDM4
- Mutant libraries
- Genetic-based screens
- Protein-protein interaction assays
- mikrobiologi
- Microbiology
Publikations- och innehållstyp
- ref (ämneskategori)
- kap (ämneskategori)
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