Expression of complement factor H binding immunoevasion proteins in Borrelia garinii isolated from patients with neuroborreliosis.

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: id:"swepub:oai:DiVA.org:umu-12936" > Expression of compl...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Alitalo, Antti (author)

Expression of complement factor H binding immunoevasion proteins in Borrelia garinii isolated from patients with neuroborreliosis.

Article/chapterEnglish2005

Publisher, publication year, extent ...

2005
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:umu-12936
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-12936URI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

The Lyme disease-pathogen Borrelia burgdorferi binds the complement inhibitor factor H (FH) to its outer surface protein E- (OspE) and BbA68-families of lipoproteins. In earlier studies, only serum-resistant strains of the genospecies B. burgdorferi sensu stricto or B. afzelii, but not serum-sensitive B. garinii strains, have been shown to bind FH. Since B. garinii often causes neuroborreliosis in man, we have readdressed the interactions of B. garinii with FH. B. garinii 50/97 strain did not express FH-binding proteins. By transforming the B. garinii 50/97 strain with an OspE-encoding gene from complement-resistant B. burgdorferi (ospE-297), its resistance to serum killing could be increased. OspE genes were detected and cloned from the B. garinii BITS, Pistoia and 40/97 strains by PCR and sequencing. The deduced amino acid sequences differed in an N-terminal lysine-rich FH-binding region from OspE sequences of resistant strains. Recombinant B. garinii BITS OspE protein was found to have a considerably lower FH-binding activity than the B. burgdorferi sensu stricto 297 OspE protein P21 (P21-297). Unlike bacteria that had been kept in culture for a long time, neurovirulent B. garinii strains from neuroborreliosis patients were found to express approximately 27-kDa FH-binding proteins. These were not recognized by polyclonal anti-OspE or anti-BbA68 antibodies. We conclude that B. garinii strains carry ospE genes but have a decreased expression of OspE proteins and a reduced ability to bind FH, especially when grown for prolonged periods in vitro. Recently isolated neuroinvasive B. garinii strains, however, can express FH-binding proteins, which may contribute to the virulence of neuroborreliosis-causing B. garinii strains.

Subject headings and genre

Amino Acid Sequence
Animals
Antigens; Bacterial/*genetics/immunology/metabolism
Bacterial Outer Membrane Proteins/*genetics/immunology/metabolism
Bacterial Proteins/*genetics/immunology/metabolism
Base Sequence
Borrelia burgdorferi Group/*pathogenicity/*physiology
Complement Factor H/immunology/metabolism
Humans
Immunoblotting
Lipoproteins/*genetics/immunology/metabolism
Lyme Neuroborreliosis/immunology/*metabolism
Molecular Sequence Data
Polymerase Chain Reaction
Surface Plasmon Resonance

Added entries (persons, corporate bodies, meetings, titles ...)

Meri, Taru (author)
Comstedt, PärUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström(Swepub:umu)parcot98 (author)
Jeffery, Luke (author)
Tornberg, Johanna (author)
Strandin, Tomas (author)
Lankinen, Hilkka (author)
Bergström, SvenUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström(Swepub:umu)svbe0001 (author)
Cinco, Marina (author)
Vuppala, Santosh R (author)
Akins, Darrin R (author)
Meri, Seppo (author)
Umeå universitetInstitutionen för molekylärbiologi (Medicinska fakulteten) (creator_code:org_t)

Related titles

In:Eur J Immunol35:10, s. 3043-30530014-2980

Internet link

Find in a library

Eur J Immunol (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Alitalo, Antti; Meri, Taru; Comstedt, Pär; Jeffery, Luke; Tornberg, Johann ...; Strandin, Tomas; show more...; Lankinen, Hilkka; Bergström, Sven; Cinco, Marina; Vuppala, Santosh ...; Akins, Darrin R; Meri, Seppo; show less...

Articles in the publication: Eur J Immunol

By the university: Umeå University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se