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Inhibition of the i...
Inhibition of the injectisome and flagellar type III secretion systems by INP1855 impairs Pseudomonas aeruginosa pathogenicity and inflammasome activation
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- Anantharajah, Ahalieyah (author)
- Pharmacologie Cellulaire et Moléculaire, Louvain Drug Research Institute, Université Catholique de Louvain, Brussels, Belgium
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- Faure, Emmanuel (author)
- EA7366, Host-Pathogen Translational Research Group, Faculté de Médecine, Université Lille Nord de France, Lille, France
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- Buyck, Julien M. (author)
- Pharmacologie Cellulaire et Moléculaire, Louvain Drug Research Institute, Université Catholique de Louvain, Brussels, Belgium; Focal Area Infection Biology, Biozentrum, University of Basel, Switzerland
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- Sundin, Charlotta (author)
- Umeå universitet,Kemiska institutionen,Creative Antibiotics, Umeå, Sweden
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- Lindmark, Tuulikki (author)
- Creative Antibiotics, Umeå, Sweden; Disruptivematerials, Uppsala, Sweden
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- Mecsas, Joan (author)
- Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, United States
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- Yahr, Timothy L. (author)
- Department of Microbiology, University of Iowa, Iowa City, United States
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- Tulkens, Paul M. (author)
- Pharmacologie Cellulaire et Moléculaire, Louvain Drug Research Institute, Université Catholique de Louvain, Brussels, Belgium
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- Mingeot-Leclercq, Marie-Paule (author)
- Pharmacologie Cellulaire et Moléculaire, Louvain Drug Research Institute, Université Catholique de Louvain, Brussels, Belgium
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- Guery, Benoît (author)
- EA7366, Host-Pathogen Translational Research Group, Faculté de Médecine, Université Lille Nord de France, Lille, France
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- Van Bambeke, Françoise (author)
- Pharmacologie Cellulaire et Moléculaire, Louvain Drug Research Institute, Université Catholique de Louvain, Brussels, Belgium
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(creator_code:org_t)
- 2016-07-13
- 2016
- English.
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In: Journal of Infectious Diseases. - : Oxford University Press. - 0022-1899 .- 1537-6613. ; 214:7, s. 1105-1116
- Related links:
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http://www.facm.ucl....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- With the rise of multidrug resistance, Pseudomonas aeruginosa infections require alternative therapeutics. The injectisome (iT3SS) and flagellar (fT3SS) type III secretion systems are 2 virulence factors associated with poor clinical outcomes. iT3SS translocates toxins, rod, needle, or regulator proteins, and flagellin into the host cell cytoplasm and causes cytotoxicity and NLRC4-dependent inflammasome activation, which induces interleukin 1 beta (IL-1 beta) release and reduces interleukin 17 (IL-17) production and bacterial clearance. fT3SS ensures bacterial motility, attachment to the host cells, and triggers inflammation. INP1855 is an iT3SS inhibitor identified by in vitro screening, using Yersinia pseudotuberculosis. Using a mouse model of P. aeruginosa pulmonary infection, we show that INP1855 improves survival after infection with an iT3SS-positive strain, reduces bacterial pathogenicity and dissemination and IL-1 beta secretion, and increases IL-17 secretion. INP1855 also modified the cytokine balance in mice infected with an iT3SS-negative, fT3SS-positive strain. In vitro, INP1855 impaired iT3SS and fT3SS functionality, as evidenced by a reduction in secretory activity and flagellar motility and an increase in adenosine triphosphate levels. As a result, INP1855 decreased cytotoxicity mediated by toxins and by inflammasome activation induced by both laboratory strains and clinical isolates. We conclude that INP1855 acts by dual inhibition of iT3SS and fT3SS and represents a promising therapeutic approach.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Keyword
- Pseudomonas aeruginosa
- type III secretion system
- flagellum
- pulmonary infection
- cytotoxicity
- flammasome activation
- anti-virulence strategy
Publication and Content Type
- ref (subject category)
- art (subject category)
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Anantharajah, Ah ...
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Faure, Emmanuel
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Buyck, Julien M.
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Sundin, Charlott ...
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Lindmark, Tuulik ...
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Mecsas, Joan
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Yahr, Timothy L.
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Tulkens, Paul M.
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