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Drop-on-demand samp...
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Fuller, Franklin DUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
(author)
Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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2017-02-27
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Macmillan Publishers Ltd.2017
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-132074
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-132074URI
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https://doi.org/10.1038/nmeth.4195DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-142628URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-320026URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Article
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X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem II, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Gul, SherazUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
(author)
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Chatterjee, RuchiraUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
(author)
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Burgie, E SetheWashington Univ St Louis, Dept Biol, St Louis, MO USA.
(author)
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Young, Iris DUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
(author)
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Lebrette, HugoStockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.(Swepub:su)hlebr
(author)
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Srinivas, VivekStockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.(Swepub:su)vsrin
(author)
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Brewster, Aaron SUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.,LCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
(author)
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Michels-Clark, TaraUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
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Clinger, Jonathan ARice Univ, Dept BioSci, Houston, TX USA.
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Andi, BabakNatl Synchrotron Light Source II, Brookhaven Natl Lab, Upton, NY USA.
(author)
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Ibrahim, MohamedHumboldt Univ, Inst Biol, Berlin, Germany.
(author)
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Pastor, ErnestUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
(author)
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de Lichtenberg, CasperUmeå universitet,Kemiska institutionen(Swepub:umu)cade0029
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Hussein, RanaHumboldt Univ, Inst Biol, Berlin, Germany.
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Pollock, Christopher JPenn State Univ, Dept Chem, University Pk, PA USA.
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Zhang, MiaoHumboldt Univ, Inst Biol, Berlin, Germany.
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Stan, Claudiu AStanford PULSE Inst, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Kroll, ThomasSSRL, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Fransson, ThomasStanford PULSE Inst, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Weninger, ClemensStanford PULSE Inst, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.;LCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
(author)
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Kubin, MarkusHelmholtz Zentrum Berlin Mat & Energie GmbH, Inst Methods & Instrumentat Synchrotron Radiat Re, Berlin, Germany.
(author)
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Aller, PierreDiamond Light Source Ltd, Harwell Sci & Innovat Campus, Didcot, Oxon, England.
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Lassalle, LouiseUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
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Brauer, PhilippDiamond Light Source Ltd, Harwell Sci & Innovat Campus, Didcot, Oxon, England.;Univ Oxford, Dept Biochem, Oxford, England.
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Miller, Mitchell DRice Univ, Dept BioSci, Houston, TX USA.
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Amin, MuhamedUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.;Zewail City Sci & Technol, Ctr Photon & Smart Mat, Giza, Egypt.
(author)
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Koroidov, SergeyUmeå universitet,Kemiska institutionen,Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA,Umea Univ, Kemiskt Biol Ctr, Inst Kemi, Umea, Sweden.;Stanford PULSE Inst, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.(Swepub:umu)seko0001
(author)
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Roessler, Christian GNatl Synchrotron Light Source II, Brookhaven Natl Lab, Upton, NY USA.;Ventana Med Syst Inc, Tucson, AZ USA.
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Allaire, MarcUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
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Sierra, Raymond GLCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Docker, Peter TDiamond Light Source Ltd, Harwell Sci & Innovat Campus, Didcot, Oxon, England.
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Glownia, James MLCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Nelson, SilkeLCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Koglin, Jason ELCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Zhu, DilingLCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Chollet, MatthieuLCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Song, Sanghoon
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Lemke, HenrikLCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.;Paul Scherrer Inst, SwissFEL, Villigen, Switzerland.
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Liang, MengningLCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Sokaras, DimosthenisSSRL, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Alonso-Mori, RobertoLCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Zouni, AthinaHumboldt Univ, Inst Biol, Berlin, Germany.
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Messinger, JohannesUppsala universitet,Umeå universitet,Kemiska institutionen,Department of Chemistry–Ångström, Molecular Biomimetics, Uppsala University, Uppsala, Sweden,Molekylär biomimetik,Umea Univ, Kemiskt Biol Ctr, Inst Kemi, Umea, Sweden.(Swepub:uu)johme269
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Bergmann, UweStanford PULSE Inst, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
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Boal, Amie KPenn State Univ, Dept Chem, University Pk, PA USA.;Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA USA.
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Bollinger Jr, J MartinPenn State Univ, Dept Chem, University Pk, PA USA.;Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA USA.
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Krebs, CarstenPenn State Univ, Dept Chem, University Pk, PA USA.;Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA USA.
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Hogbom, MartinStanford Univ, Dept Chem, Stanford, CA USA.
(author)
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Phillips Jr, George NRice Univ, Dept BioSci, Houston, TX USA.;Rice Univ, Dept Chem, Houston, TX USA.
(author)
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Vierstra, Richard DWashington Univ St Louis, Dept Biol, St Louis, MO USA.
(author)
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Sauter, Nicholas KUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
(author)
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Orville, Allen MDiamond Light Source Ltd, Harwell Sci & Innovat Campus, Didcot, Oxon, England.
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Kern, JanUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.;LCLS, SLAC Natl Accelerator Lab, Menlo Pk, CA USA.
(author)
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Yachandra, Vittal KUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
(author)
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Yano, JunkoUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
(author)
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Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.Washington Univ St Louis, Dept Biol, St Louis, MO USA.
(creator_code:org_t)
Related titles
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In:Nature Methods: Macmillan Publishers Ltd.14, s. 443-4491548-70911548-7105
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- By the author/editor
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Fuller, Franklin ...
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Gul, Sheraz
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Chatterjee, Ruch ...
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Burgie, E Sethe
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Young, Iris D
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Lebrette, Hugo
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show more...
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Srinivas, Vivek
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Brewster, Aaron ...
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Michels-Clark, T ...
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Clinger, Jonatha ...
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Andi, Babak
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Ibrahim, Mohamed
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Pastor, Ernest
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de Lichtenberg, ...
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Hussein, Rana
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Pollock, Christo ...
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Zhang, Miao
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Stan, Claudiu A
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Kroll, Thomas
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Fransson, Thomas
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Weninger, Clemen ...
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Kubin, Markus
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Aller, Pierre
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Lassalle, Louise
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Brauer, Philipp
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Miller, Mitchell ...
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Amin, Muhamed
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Koroidov, Sergey
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Roessler, Christ ...
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Allaire, Marc
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Sierra, Raymond ...
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Docker, Peter T
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Glownia, James M
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Nelson, Silke
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Koglin, Jason E
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Zhu, Diling
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Chollet, Matthie ...
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Song, Sanghoon
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Lemke, Henrik
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Liang, Mengning
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Sokaras, Dimosth ...
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Alonso-Mori, Rob ...
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Zouni, Athina
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Messinger, Johan ...
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Bergmann, Uwe
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Boal, Amie K
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Bollinger Jr, J ...
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Krebs, Carsten
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Hogbom, Martin
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Phillips Jr, Geo ...
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Vierstra, Richar ...
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Sauter, Nicholas ...
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Orville, Allen M
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Kern, Jan
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Yachandra, Vitta ...
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Yano, Junko
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Umeå University
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Stockholm University
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