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Hydrophilic Separat...
Hydrophilic Separation Materials for Liquid Chromatography
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- Hemström, Petrus, 1968- (author)
- Umeå universitet,Kemiska institutionen
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- Irgum, Knut, Prof. (thesis advisor)
- Umeå universitet,Kemiska institutionen
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- Schoenmakers, Peter, Prof. Dr. ir. (opponent)
- Universiteit van Amsterdam
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(creator_code:org_t)
- ISBN 9789172644069
- Umeå : Kemi, 2007
- English 24 s.
- Related links:
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https://umu.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- The main focus of this thesis is on hydrophilic interaction chromatography (HILIC) and the preparation of stationary phases for HILIC. The mechanism of HILIC is also discussed; a large part of the discussion has been adapted from a review written by me and professor Irgum for the Journal of Separation Science (ref 34). By reevaluating the literature we have revealed that the notion of HILIC as simply partitioning chromatography needed modification. However, our interest in the HILIC mechanism was mainly inspired by the need to understand how to construct the optimal HILIC stationary phase. The ultimate stationary phase for HILIC is still not found. My theory is that a non-charged stationary phase capable of retaining a full hydration layer even at extreme acetonitrile (> 85%) concentrations should give a HILIC stationary phase with a more pure partitioning retention behavior similar to that of a swollen C18 reversed phase. The preparation of a sorbitol methacrylate grafted silica stationary phase is one of our attempts at producing such a stationary phase. The preparation of such a grafted silica has been performed, but with huge difficulty and this work is still far from producing a column of commercial quality and reprodicibility. This thesis also discusses a new method for the initiation of atom transfer radical polymerization from chlorinated silica. This new grafting scheme theoretically results in a silica particle grafted with equally long polymer chains, anchored to the silica carrier by a hydrolytically stable silicon-carbon bond. The hydrolytic stability is especially important for HILIC stationary phases due to the high water concentration at the surface.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Chromatography
- HILIC
- Hydrophilic
- Stationary phase
- Chemistry
- Kemi
Publication and Content Type
- vet (subject category)
- dok (subject category)
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