WFRF:(Larsen Michael)
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- Kessel, Line (author)
Impact of UVR-A on whole human lenses, supernatants of buffered human lens homogenates, and purified argpyrimidine and 3-OH-kynurenine
- Article/chapterEnglish2005
Publisher, publication year, extent ...
- 2005-03-02
- Wiley,2005
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:umu-13526
- https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-13526URI
- https://doi.org/10.1111/j.1600-0420.2005.00388.xDOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- Purpose: Yellow chromophores and fluorescent compounds accumulate in the lens with age. Some of these compounds are photochemically active. The present study aimed to examine the photochemical effect of ultraviolet radiation-A (UVR-A) on the human lens.Methods: Intact human lenses and supernatants of buffered lens homogenates were exposed to UVR-A. The effect of UVR-A was evaluated by time-resolved and steady-state fluorescence spectroscopy, visual evaluation of colour and protein gel electrophoresis.Results: Intact lenses exposed to UVR-A showed no changes in time-resolved or steady-state fluorescence properties but the yellow coloration was visibly attenuated. The supernatants of buffered lens homogenates exposed to UVR-A demonstrated a reduction in time-resolved and steady-state fluorescent properties and protein cross-linking.Conclusions: Exposure of the intact lens to UVR-A causes chromophore bleaching without affecting fluorescence, indicating that non-fluorescent chromophores have been destroyed. After homogenization, both chromophores and fluorophores from the lens suffer damage and proteins aggregate. This indicates that powerful mechanisms of protection against UVR-A found in the intact lens are disturbed by homogenization of the lens, suggesting that isolated lens proteins cannot be used as a model system for studying cataractogenesis. Hypothetically, the protective mechanism could be related to the rigidly packed three-dimensional structure of the lens proteins or to the abundance of antioxidative and free radical scavenging defence systems.
Subject headings and genre
- cataract
- fluorescence
- lens
- ultraviolet radiation
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- Kalinin, StanislavUmeå universitet,Kemiska institutionen (author)
- Soroka, Vladislav (author)
- Larsen, Michael (author)
- Johansson, Lennart B-ÅUmeå universitet,Kemiska institutionen(Swepub:umu)lejo0007 (author)
- Umeå universitetKemiska institutionen (creator_code:org_t)
Related titles
- In:ACTA OPHTHALMOLOGICA SCANDINAVICA: Wiley83:2, s. 221-71395-3907
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