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Lack of the light-h...
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Kovács, LaszloDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
(författare)
Lack of the light-harvesting complex CP24 affects the structure and function of the grana membranes of higher plant chloroplasts.
- Artikel/kapitelEngelska2006
Förlag, utgivningsår, omfång ...
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2006-11-17
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Oxford University Press (OUP),2006
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:umu-14197
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-14197URI
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https://doi.org/10.1105/tpc.106.045641DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The photosystem II (PSII) light-harvesting antenna in higher plants contains a number of highly conserved gene products whose function is unknown. Arabidopsis thaliana plants depleted of one of these, the CP24 light-harvesting complex, have been analyzed. CP24-deficient plants showed a decrease in light-limited photosynthetic rate and growth, but the pigment and protein content of the thylakoid membranes were otherwise almost unchanged. However, there was a major change in the macroorganization of PSII within these membranes; electron microscopy and image analysis revealed the complete absence of the C2S2M2 light-harvesting complex II (LHCII)/PSII supercomplex predominant in wild-type plants. Instead, only C2S2 supercomplexes, which are deficient in the LHCIIb M-trimers, were found. Spectroscopic analysis confirmed the disruption of the wild-type macroorganization of PSII. It was found that the functions of the PSII antenna were disturbed: connectivity between PSII centers was reduced, and maximum photochemical yield was lowered; rapidly reversible nonphotochemical quenching was inhibited; and the state transitions were altered kinetically. CP24 is therefore an important factor in determining the structure and function of the PSII light-harvesting antenna, providing the linker for association of the M-trimer into the PSII complex, allowing a specific macroorganization that is necessary both for maximum quantum efficiency and for photoprotective dissipation of excess excitation energy.
Ämnesord och genrebeteckningar
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Arabidopsis/*metabolism/radiation effects/ultrastructure
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Arabidopsis Proteins/analysis/isolation & purification/*metabolism
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Chloroplasts/*ultrastructure
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Chromatography; Gel
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Circular Dichroism
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DNA; Bacterial/metabolism
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Fluorescence
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Light
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Light-Harvesting Protein Complexes/analysis/*deficiency/isolation & purification
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Models; Biological
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Mutagenesis; Insertional
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Photosynthesis/radiation effects
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Photosynthetic Reaction Center Complex Proteins/metabolism
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Photosystem II Protein Complex/metabolism
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Pigments; Biological/metabolism
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Plant Leaves/radiation effects
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RNA; Antisense/metabolism
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Structure-Activity Relationship
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Temperature
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Thylakoids/*ultrastructure
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Damkjaer, Jakob,1976-Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)(Swepub:umu)jatdar04
(författare)
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Kereïche, SamiDepartment of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
(författare)
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Ilioaia, CristianDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
(författare)
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Ruban, Alexander VDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
(författare)
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Boekema, Egbert JDepartment of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
(författare)
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Jansson, Stefan,1959-Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)(Swepub:umu)stja0001
(författare)
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Horton, PeterDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
(författare)
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Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United KingdomInstitutionen för fysiologisk botanik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:The Plant Cell: Oxford University Press (OUP)18:11, s. 3106-201040-46511532-298X
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Kovács, Laszlo
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Damkjaer, Jakob, ...
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Kereïche, Sami
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Ilioaia, Cristia ...
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Ruban, Alexander ...
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Boekema, Egbert ...
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Jansson, Stefan, ...
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Horton, Peter
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- Artiklar i publikationen
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The Plant Cell
- Av lärosätet
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Umeå universitet