SwePub
Sök i LIBRIS databas

  Utökad sökning

id:"swepub:oai:DiVA.org:umu-143601"
 

Sökning: id:"swepub:oai:DiVA.org:umu-143601" > Fos Promotes Early ...

  • Chen, JialinUmeå universitet,Anatomi,Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, Zhejiang; Zhejiang Provincial Key Lab forTissue Engineering and Regenerative Medicine, Hangzhou, Zhejiang, People’s Republic of China (författare)

Fos Promotes Early Stage Teno-Lineage Differentiation of Tendon Stem/Progenitor Cells in Tendon

  • Artikel/kapitelEngelska2017

Förlag, utgivningsår, omfång ...

  • 2017-10-10
  • John Wiley & Sons,2017
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:umu-143601
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-143601URI
  • https://doi.org/10.1002/sctm.15-0146DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Stem cells have been widely used in tendon tissue engineering. The lack of refined and controlled differentiation strategy hampers the tendon repair and regeneration. This study aimed to find new effective differentiation factors for stepwise tenogenic differentiation. By microarray screening, the transcript factor Fos was found to be expressed in significantly higher amounts in postnatal Achilles tendon tissue derived from 1 day as compared with 7-days-old rats. It was further confirmed that expression of Fos decreased with time in postnatal rat Achilles tendon, which was accompanied with the decreased expression of multiply tendon markers. The expression of Fos also declined during regular in vitro cell culture, which corresponded to the loss of tendon phenotype. In a cell-sheet and a three-dimensional cell culture model, the expression of Fos was upregulated as compared with in regular cell culture, together with the recovery of tendon phenotype. In addition, significant higher expression of tendon markers was found in Fos-overexpressed tendon stem/progenitor cells (TSPCs), and Fos knock-down gave opposite results. In situ rat tendon repair experiments found more normal tendon-like tissue formed and higher tendon markers expression at 4 weeks postimplantation of Fos-overexpressed TSPCs derived nonscaffold engineering tendon (cell-sheet), as compared with the control group. This study identifies Fos as a new marker and functional driver in the early stage teno-lineage differentiation of tendon, which paves the way for effective stepwise tendon differentiation and future tendon regeneration.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Zhang, Erchen (författare)
  • Zhang, Wei (författare)
  • Liu, Zeyu (författare)
  • Lu, Ping (författare)
  • Zhu, Ting (författare)
  • Yin, Zi (författare)
  • Backman, Ludvig J.Umeå universitet,Anatomi(Swepub:umu)lugban04 (författare)
  • Liu, Huanhuan (författare)
  • Chen, Xiao (författare)
  • Ouyang, Hongwei (författare)
  • Umeå universitetAnatomi (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Stem Cells Translational Medicine: John Wiley & Sons6:11, s. 2009-20192157-65642157-6580

Internetlänk

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy