SNX18 regulates ATG9A trafficking from recycling endosomes by recruiting Dynamin-2

Trafficking of mammalian ATG9A between the Golgi apparatus, endosomes and peripheral ATG9A compartments is important for autophagosome biogenesis. Here, we show that the membrane remodelling protein SNX18, previously identified as a positive regulator of autophagy, regulates ATG9A trafficking from recycling endosomes. ATG9A is recruited to SNX18-induced tubules generated from recycling endosomes and accumulates in juxtanuclear recycling endosomes in cells lacking SNX18. Binding of SNX18 to Dynamin-2 is important for ATG9A trafficking from recycling endosomes and for formation of ATG16L1- and WIPI2-positive autophagosome precursor membranes. We propose a model where upon autophagy induction, SNX18 recruits Dynamin-2 to induce budding of ATG9A and ATG16L1 containing membranes from recycling endosomes that traffic to sites of autophagosome formation.

Munson, Michael J. (author)
Lamb, Christopher A. (author)
Bjørndal, Gunnveig T. (author)
Pankiv, Serhiy (author)
Carlsson, Sven R.Umeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)svca0001 (author)
Tooze, Sharon A. (author)
Simonsen, Anne (author)
Umeå universitetInstitutionen för medicinsk kemi och biofysik (creator_code:org_t)

By the author/editor: Søreng, Kristian ...; Munson, Michael ...; Lamb, Christophe ...; Bjørndal, Gunnve ...; Pankiv, Serhiy; Carlsson, Sven R ...; show more...; Tooze, Sharon A.; Simonsen, Anne; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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