PTE, a novel module to target Polycomb Repressive Complex 1 to the human cyclin D2 (CCND2) oncogene

Cameron, Sarina R.Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten) (author)

Polycomb group proteins are essential epigenetic repressors. They form multiple protein complexes of which two kinds, PRC1 and PRC2, are indispensable for repression. Although much is known about their biochemical properties, how mammalian PRC1 and PRC2 are targeted to specific genes is poorly understood. Here, we establish the cyclin D2 (CCND2) oncogene as a simple model to address this question. We provide the evidence that the targeting of PRC1 to CCND2 involves a dedicated PRC1-targeting element (PTE). The PTE appears to act in concert with an adjacent cytosine-phosphate-guanine (CpG) island to arrange for the robust binding of PRC1 and PRC2 to repressed CCND2. Our findings pave the way to identify sequence-specific DNA-binding proteins implicated in the targeting of mammalian PRC1 complexes and provide novel link between polycomb repression and cancer.

Nandi, SoumyadeepUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)(Swepub:umu)sona0014 (author)
Kahn, Tatyana G.Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)(Swepub:umu)taka0009 (author)
Barrasa, Juan I.Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)(Swepub:umu)juig0002 (author)
Stenberg, Per,1974-Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Division of Chemical, Biological, Radioactive and Nuclear (CBRN) Security and Defence, FOI–Swedish Defence Research Agency, 906 21 Umeå Sweden(Swepub:umu)perstg96 (author)
Schwartz, Yuri B.Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)(Swepub:umu)yusc0002 (author)
Umeå universitetInstitutionen för molekylärbiologi (Medicinska fakulteten) (creator_code:org_t)

In:Journal of Biological Chemistry: American Society for Biochemistry and Molecular Biology293:37, s. 14342-143580021-92581083-351X

By the author/editor: Cameron, Sarina ...; Nandi, Soumyadee ...; Kahn, Tatyana G.; Barrasa, Juan I.; Stenberg, Per, 1 ...; Schwartz, Yuri B ...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Medical Genetics

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