SwePub
Sök i LIBRIS databas

  Extended search

id:"swepub:oai:DiVA.org:umu-152536"
 

Search: id:"swepub:oai:DiVA.org:umu-152536" > Detection of micro-...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Zhang, Lu (author)

Detection of micro-metastases by flow cytometry in lymph nodes from patients with penile cancer

  • Article/chapterEnglish2018

Publisher, publication year, extent ...

  • 2018-10-05
  • BioMed Central (BMC),2018
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:umu-152536
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-152536URI
  • https://doi.org/10.1186/s12894-018-0399-3DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:139335429URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • BACKGROUND: The tumor draining lymph node concept was first described in penile cancer for staging. Immunohistochemistry and histopathology evaluations are routinely used in clinical practice to examine lymph nodes for metastasis. However, these methods are time-consuming with low diagnostic accuracy and micro-metastases might be missed. In this study, we aim to evaluate detection of metastatic cells in draining lymph nodes by flow cytometry.METHODS: To assess the sensitivity of micro-metastasis detection by FACS (Fluorescence-activated cell sorting), HeLa cells were titrated into Peripheral blood mononuclear cells (PBMCs) and expression of pan-cytokeratin AE1/AE3 was analyzed. Single cell suspensions were separately prepared from 10 regional lymph nodes obtained from 5 patients with invasive penile cancer undergoing radical surgery and lymph node dissection. Lymph node dereived cells were examined for cell surface expression of EpCAM, E-cadherin and intracellular expression of pan-cytokeratin AE1/AE3 by FACS.RESULTS: Ten lymph nodes from 5 penile cancer patients were investigated in a head-to-head comparison between FACS and pathology examination of sections. All metastatic lymph nodes verified by pathology examination were also identified by FACS. Two additional lymph nodes with micro-metastases were diagnosed by FACS only.CONCLUSIONS: FACS analyses of pan-cytokeratin AE1/AE3 stained single cells from tumor draining lymph nodes can be used to detect micro-metastases in patients with penile cancer patients.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Hu, Jin (author)
  • Zirakzadeh, A. AliUmeå universitet,Urologi och andrologi,Department of Medicine, Immunology and Allergy Unit, Karolinska University Hospital, Stockholm, Sweden(Swepub:umu)alizih97 (author)
  • Rosvall, JesperKarolinska Institutet (author)
  • Hedlund, Mats (author)
  • Hu, Ping Sheng (author)
  • P A Wallin, Robert (author)
  • Sherif, AmirUmeå universitet,Urologi och andrologi(Swepub:umu)amsh0008 (author)
  • Winqvist, Ola (author)
  • Umeå universitetUrologi och andrologi (creator_code:org_t)

Related titles

  • In:BMC Urology: BioMed Central (BMC)18:11471-2490

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view