A standardized protocol for comparable analysis of GSH/GSSG by UHPLC-ESI-MSMS for human plasma

Claeson, Anna-Sara,1974-Umeå universitet,Institutionen för psykologi (författare)

Variability in the levels of GSH and GSSG in plasma is suggested to derive from inadequate pre-processing methods. The aim of this study was to develop a protocol for comparable and reliable measurements of GSH/GSSG. Venous blood from 8 healthy individuals were collected and divided into 7 different pre-processing procedures. For three of the samples an extraction mixture was added after 0 (baseline), 4 and 8 min and for three of the samples the extraction mixture was added at different times during defrost. A worst case scenario where a sample was left in a cool box during 6 h was also included. The samples were analyzed with UHPLC-ESIMSMS. A large difference in the levels of GSH and GSSG were identified and it was clearly associated with the sample handling procedures. A sample left untreated for 4 min will have significantly reduced amount of GSH. Stability tests showed that the level of GSH was reduced after 3 months in -80 degrees C.

Gouveia-Figueira, Sandra C.Swedish Metabolomics Centre (SMC), Umeå, Sweden(Swepub:umu)sago0010 (författare)
Stenlund, HansSwedish Metabolomics Centre (SMC), Umeå, Sweden(Swepub:umu)hasstd97 (författare)
Johansson, Annika I.Swedish Metabolomics Centre (SMC), Umeå, Sweden (författare)
Umeå universitetInstitutionen för psykologi (creator_code:org_t)

Av författaren/redakt...: Claeson, Anna-Sa ...; Gouveia-Figueira ...; Stenlund, Hans; Johansson, Annik ...

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