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Urea dilution of se...
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Olsson, JanUmeå universitet,Virologi,Umeå Univ, Dept Clin Microbiol, Virol, Umeå, Sweden
(författare)
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
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2019-02-14
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BioMed Central,2019
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:umu-157213
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-157213URI
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https://doi.org/10.1186/s12879-019-3769-xDOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-378996URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900-0.979) and 0.989 (95% CI 0.969-0.996) in the biobank and clinical samples, respectively. The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts.
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Johansson, JörgenUmeå universitet,Virologi,Laboratory Medicine, Clinical Microbiology, Umeå University Hospital, Umeå, Sweden,Umeå Univ, Dept Clin Microbiol, Umeå, Sweden; Umeå Univ Hosp, Lab Med, Clin Microbiol, Umeå, Sweden(Swepub:umu)jojo0001
(författare)
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Honkala, EmmaUmeå universitet,Virologi,Umeå Univ, Dept Clin Microbiol, Umeå, Sweden(Swepub:umu)emho0009
(författare)
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Blomqvist, BertUmeå universitet,Virologi,Laboratory Medicine, Clinical Microbiology, Umeå University Hospital, Umeå, Sweden,Umeå Univ, Dept Clin Microbiol, Umeå, Sweden; Umeå Univ Hosp, Lab Med, Clin Microbiol, Umeå, Sweden
(författare)
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Kok, EloiseTampere Univ Hosp, Tampere, Finland
(författare)
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Weidung, BodilUppsala universitet,Geriatrik(Swepub:uu)bodwe750
(författare)
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Lövheim, Hugo,1981-Umeå universitet,Geriatrik,Umeå Univ, Community Med & Rehabil, Geriatr Med, Umeå, Sweden(Swepub:umu)huolom01
(författare)
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Elgh, Fredrik,1957-Umeå universitet,Virologi,Umeå Univ, Dept Clin Microbiol, Umeå, Sweden(Swepub:umu)frkelh76
(författare)
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Umeå universitetVirologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:BMC Infectious Diseases: BioMed Central191471-2334
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Olsson, Jan
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Johansson, Jörge ...
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Honkala, Emma
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Blomqvist, Bert
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Kok, Eloise
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Weidung, Bodil
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Lövheim, Hugo, 1 ...
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Elgh, Fredrik, 1 ...
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