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- Chandra, Naresh,1987-Umeå universitet,Avdelningen för virologi,Arnberg (author)
Sialic Acid-Containing Glycans as Cellular Receptors for Ocular Human Adenoviruses : Implications for Tropism and Treatment
- Article/chapterEnglish2019
Publisher, publication year, extent ...
- 2019-04-27
- MDPI,2019
- electronicrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:umu-159268
- https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-159268URI
- https://doi.org/10.3390/v11050395DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Human adenoviruses (HAdV) are the most common cause of ocular infections. Species B human adenovirus type 3 (HAdV-B3) causes pharyngoconjunctival fever (PCF), whereas HAdV-D8, -D37, and -D64 cause epidemic keratoconjunctivitis (EKC). Recently, HAdV-D53, -D54, and -D56 emerged as new EKC-causing agents. HAdV-E4 is associated with both PCF and EKC. We have previously demonstrated that HAdV-D37 uses sialic acid (SA)-containing glycans as cellular receptors on human corneal epithelial (HCE) cells, and the virus interaction with SA is mediated by the knob domain of the viral fiber protein. Here, by means of cell-based assays and using neuraminidase (a SA-cleaving enzyme), we investigated whether ocular HAdVs other than HAdV-D37 also use SA-containing glycans as receptors on HCE cells. We found that HAdV-E4 and -D56 infect HCE cells independent of SAs, whereas HAdV-D53 and -D64 use SAs as cellular receptors. HAdV-D8 and -D54 fiber knobs also bound to cell-surface SAs. Surprisingly, HCE cells were found resistant to HAdV-B3 infection. We also demonstrated that the SA-based molecule i.e., ME0462, designed to bind to SA-binding sites on the HAdV-D37 fiber knob, efficiently prevents binding and infection of several EKC-causing HAdVs. Surface plasmon resonance analysis confirmed a direct interaction between ME0462 and fiber knobs. Altogether, we demonstrate that SA-containing glycans serve as receptors for multiple EKC-causing HAdVs, and, that SA-based compound function as a broad-spectrum antiviral against known and emerging EKC-causing HAdVs.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
- adenovirus
- cellular receptor
- epidemic keratoconjunctivitis
- pharyngoconjunctival fever
- sialic acid
- tropism
Added entries (persons, corporate bodies, meetings, titles ...)
- Frängsmyr, LarsUmeå universitet,Avdelningen för virologi,Arnberg(Swepub:umu)lacfrr79 (author)
- Imhof, SophieUmeå universitet,Avdelningen för virologi(Swepub:umu)soim0002 (author)
- Caraballo, RémiUmeå universitet,Kemiska institutionen(Swepub:umu)reca0008 (author)
- Elofsson, MikaelUmeå universitet,Kemiska institutionen(Swepub:umu)miel0001 (author)
- Arnberg, Niklas,1987-Umeå universitet,Avdelningen för virologi,Arnberg(Swepub:umu)niar0001 (author)
- Umeå universitetAvdelningen för virologi (creator_code:org_t)
Related titles
- In:Viruses: MDPI11:51999-4915
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