Metabolic profiling of zebrafish embryo development from blastula period to early larval stages

Dhillon, Sundeep S.Univ Birmingham, Coll Med & Dent Sci, Sch Clin & Expt Med, Birmingham, W Midlands, England (författare)

The zebrafish embryo is a popular model for drug screening, disease modelling and molecular genetics. In this study, samples were obtained from zebrafish at different developmental stages. The stages that were chosen were 3/4, 4/5, 24, 48, 72 and 96 hours post fertilization (hpf). Each sample included fifty embryos. The samples were analysed using gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). Principle component analysis (PCA) was applied to get an overview of the data and orthogonal projection to latent structure discriminant analysis (OPLS-DA) was utilised to discriminate between the developmental stages. In this way, changes in metabolite profiles during vertebrate development could be identified. Using a GC-TOF-MS metabolomics approach it was found that nucleotides and metabolic fuel (glucose) were elevated at early stages of embryogenesis, whereas at later stages amino acids and intermediates in the Krebs cycle were abundant. This agrees with zebrafish developmental biology, as organs such as the liver and pancreas develop at later stages. Thus, metabolomics of zebrafish embryos offers a unique opportunity to investigate large scale changes in metabolic processes during important developmental stages in vertebrate development. In terms of stability of the metabolic profile and viability of the embryos, it was concluded at 72 hpf was a suitable time point for the use of zebrafish as a model system in numerous scientific applications.

Torell, FridaUmeå universitet,Kemiska institutionen,Accelerator Lab (ACL), Karlsruhe Institute of Technology, Karlsruhe, Germany; AcureOmics, Umeå, Sweden,Umea Univ, Dept Chem, Computat Life Sci Cluster CLiC, Umea, Sweden;Karlsruhe Inst Technol, Accelerator Lab ACL, Karlsruhe, Germany;AcureOmics, Umea, Sweden(Swepub:umu)frto0004 (författare)
Donten, MagdalenaAcureOmics, Umea, Sweden (författare)
Lundstedt-Enkel, Katrin,1959-Uppsala universitet,Miljötoxikologi,AcureOmics, Umea, Sweden(Swepub:uu)kalun030 (författare)
Bennett, KateAcureOmics, Umea, Sweden (författare)
Raennar, StefanAcureOmics, Umea, Sweden;Sartorius AG, Corp Res, Gottingen, Germany (författare)
Trygg, JohanUmeå universitet,Kemiska institutionen,AcureOmics, Umeå, Sweden,Umea Univ, Dept Chem, Computat Life Sci Cluster CLiC, Umea, Sweden;Sartorius AG, Corp Res, Gottingen, Germany(Swepub:umu)jotr0001 (författare)
Lundstedt, TorbjörnUppsala universitet,Institutionen för farmaceutisk biovetenskap,AcureOmics, Umea, Sweden(Swepub:uu)tolun677 (författare)
Univ Birmingham, Coll Med & Dent Sci, Sch Clin & Expt Med, Birmingham, W Midlands, EnglandKemiska institutionen (creator_code:org_t)

Av författaren/redakt...: Dhillon, Sundeep ...; Torell, Frida; Donten, Magdalen ...; Lundstedt-Enkel, ...; Bennett, Kate; Raennar, Stefan; visa fler...; Trygg, Johan; Lundstedt, Torbj ...; visa färre...

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