Screening for Differentially Expressed Circular RNAs in the Cartilage of Osteoarthritis Patients for Their Diagnostic Value

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Wang, YingDepartment of Orthopedics, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an, People's Republic of China (författare)

Screening for Differentially Expressed Circular RNAs in the Cartilage of Osteoarthritis Patients for Their Diagnostic Value

Artikel/kapitelEngelska2019

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Mary Ann Liebert,2019
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LIBRIS-ID:oai:DiVA.org:umu-163635
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-163635URI
https://doi.org/10.1089/gtmb.2019.0108DOI

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Språk:engelska
Sammanfattning på:engelska

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Background: Osteoarthritis (OA) is the most prevalent osteoarticular disease, which typically involves chronic cartilage degeneration and synovitis. The latest research shows that circular RNAs (circRNAs) play a role in the development of a variety of diseases, including osteoarthrosis.Purposes: The aim of this study was to explore the expression of circRNAs in OA chondrocytes and predict biomarkers for diagnosis.Materials and Methods: The circRNA expression profile was analyzed through use of the Gene Spring software V13.0; differentially expressed circRNAs were screened by comparing OA chondrocytes and normal articular chondrocytes. We validated the microarray data by quantitative real-time polymerase chain reaction analyses of OA chondrocytes and chondrocytes from normal controls. TargetScan software and miRanda software were used to predict networks of circRNA–miRNA interactions in cartilage. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) analyses were applied to predict the functions of differentially expressed circRNAs.Results: Overall, 1380 circRNAs were differentially expressed between OA chondrocytes and normal articular chondrocytes (fold-change ≥2, p ≤ 0.05), including 215 that were upregulated and 1165 that were downregulated circRNAs. After analyzing the differentially expressed circRNA genes, the top 20 enriched GO entries and KEGG pathways were annotated. The hsa_circrna_0032131 was identified for further analysis. A circRNA–miRNA network was constructed to represent the 10 most likely target genes associated with the validated circRNA.Conclusions: Our research suggests that some of the differentially expressed circRNAs in OA chondrocytes compared to normal chondrocytes are etiologically associated with the pathological process of OA. It was found that hsa_circRNA_0032131 likely participates in the initiation and progression of OA and has potential as a diagnostic marker.Clinical Relevance: To analyze the difference of circRNA expression profiles between OA and normal controls and explore biomarkers for diagnosis.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Ortopedi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Orthopaedics hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Reumatologi och inflammation hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Rheumatology and Autoimmunity hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Biomedicinsk laboratorievetenskap/teknologi hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Biomedical Laboratory Science/Technology hsv//eng
osteoarthritis
circRNAs
expression profile
functional analysis
molekylärbiologi
Molecular Biology
Clinical Chemistry
klinisk kemi
Orthopaedics
ortopedi
reumatologi
rheumatology
medicinsk biokemi
Medical Biochemistry

Biuppslag (personer, institutioner, konferenser, titlar ...)

Wu, CuiyanSchool of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China (författare)
Zhang, FengSchool of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China (författare)
Zhang, YananSchool of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China (författare)
Ren, ZhiweiDepartment of Orthopedics, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an, People's Republic of China (författare)
Lammi, Mikko J.,1961-Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),School of Public Health, Xi'an Jiaotong University Health Science Center; Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China,Chondrogen ic and Osteogenic Differentiation(Swepub:umu)mila0077 (författare)
Guo, XiongSchool of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China (författare)
Department of Orthopedics, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an, People's Republic of ChinaSchool of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China (creator_code:org_t)

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Ingår i:Genetic Testing and Molecular Biomarkers: Mary Ann Liebert23:10, s. 706-7161945-02651945-0257

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Av författaren/redakt...: Wang, Ying; Wu, Cuiyan; Zhang, Feng; Zhang, Yanan; Ren, Zhiwei; Lammi, Mikko J., ...; visa fler...; Guo, Xiong; visa färre...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

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