Fyn binding protein, Fyb, interacts with mammalian actin binding protein, mAbp1.

• The immune cell specific protein Fyn-T binding protein (Fyb) has been identified as a target of the Yersinia antiphagocytic effector Yersinia outer protein H (YopH), but its role in macrophages is unknown. By using Fyb domains as bait to screen a mouse lymphoma cDNA library, we identified a novel interaction partner, mammalian actin binding protein 1 (mAbp1). We show that mAbp1 binds the Fyb N-terminal via its C-terminally located src homology 3 domain. The interaction between Fyb and mAbp1 is detected in macrophage lysates and the proteins co-localize with F-actin in the leading edge. Hence, mAbp1 is likely to constitute a downstream effector of Fyb involved in F-actin dynamics.

Nyckelord

Actins/metabolism

Adaptor Proteins; Signal Transducing/chemistry/genetics/*metabolism

Amino Acid Motifs

Animals

Bacterial Outer Membrane Proteins/metabolism

Cell Line

Mice

Microfilament Proteins/genetics/*metabolism

Phosphorylation

Phosphotyrosine/metabolism

Protein Binding

Protein Transport

Protein-Tyrosine-Phosphatase/metabolism

Proto-Oncogene Proteins/metabolism

Proto-Oncogene Proteins c-fyn

Two-Hybrid System Techniques

src Homology Domains/genetics

src-Family Kinases/metabolism

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)