Microscopic and spectroscopic characterization of rice and corn starch

• Starch granules from rice and corn were isolated, and their molecular mechanism on interaction with alpha-amylase was characterized through biochemical test, microscopic imaging, and spectroscopic measurements. The micro-scale structure of starch granules were observed under an optical microscope and their average size was in the range 1-100 mu m. The surface topological structures of starch with micro-holes due to the effect of alpha- amylase were also visualized under scanning electron microscope. The crystallinity was confirmed by X-ray diffraction patterns as well as second-harmonic generation microscopy. The change in chemical bonds before and after hydrolysis of the starch granules by alpha- amylase was determined by Fourier transform infrared spectroscopy. Combination of microscopy and spectroscopy techniques relates structural and chemical features that explain starch enzymatic hydrolysis which will provide a valid basis for future studies in food science and insights into the energy transformation dynamics.

Ämnesord

NATURVETENSKAP  -- Kemi -- Materialkemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Materials Chemistry (hsv//eng)

Nyckelord

Fourier transform infrared spectroscopy

optical microscope

scanning electron microscope

starch

X-ray diffraction

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)